biologia plantarum

Biologia plantarum 33:298-302, 1991 | DOI: 10.1007/BF02885377

Residual Nuclear structures fromZea mays L.

Zoya Galcheva-Gargova¹, Snezhana Doncheva², Elena Marinova³, Stanka Koleva³

¹ Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia, Bulgaria

² Institute of Plant Physiology, Bulgarian Academy of Sciences, Sofia, Bulgaria

³ Institute of Genetic Engineering, Agricultural Academy, Kostinbrod-2, Bulgaria

Nuclei fromZea mays L. root tip meristematic cells were treated according to the conventional method for nuclear matrix isolation and according to a recently adapted procedure for isolation of nuclear shells from plant cells. In the first case, after high salt extraction of proteins and DNase I and RNase digestions, residual structures are obtained consisting of a periferal layer and an internal network. The obtained structures are very similar to the nuclear matrix preparations from animal cells. In case nuclei are swollen in EDTA first, digested with DNase II and RNase prior high salt treatment, structures devoid of internal network are obtained. The analogous residual structures were shown forPhaseolus vulgaris L. meristematic root cells nuclei (Galcheva-Gargovaet al. 1988). The morphology and the protein composition of the two types of residual structures suggest that the organization of scaffold structures from plant nuclei is very similar to the one from animal cell nuclei.

Keywords: Zea mays L; internal nuclear matrix; nuclear shells; residual proteins

Received: June 26, 1989; Accepted: March 16, 1990; Published: July 1, 1991  Show citation