biologia plantarum

Biologia plantarum 33:433, 1991 | DOI: 10.1007/BF02897714

Transitory pulse-like treatment with IAA solution more effectively induces xylogenesis in callus culture than permanent presence of the auxin

Wieslawa Kuternozińska¹, Marta Pilipowicz¹, W. Korohoda²

¹ Laboratory of Cytophysiology, Department of Plant Physiology, Polish Academy of Sciences, Cracow, Polland

² The Jan Zurzycki Institute of Molecular Biology, Jagiellonian University, Cracow, Poland

Differentiation of Acerpseudoplatanus L. cells into tracheary elements (TE) and an increase in the number of TE inHaplopappus gracilis (Nutt.) A. Gray calli were observed after pulse-treatment of the cultures grown in glass tubes with indole-3-acetic acid (IAA) solutions. The effect was enhanced if the treatment was repeated in three subsequent days. The manner of IAA application which caused a wave-like pattern of IAA flow through the callus culture appeared to be more important than the IAA concentration. Induction of differentiation of A.pseudoplatanus cells and an increase in the number of TE inH. gracilis callus did not occur when the calli were grown on agar media supplemented with increased concentrations of IAA.

Received: September 3, 1990; Accepted: November 12, 1990; Published: November 1, 1991  Show citation