biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 34:213-218, 1992 | DOI: 10.1007/BF02925871

Somatic embryogenesis in European black pine (Pinus nigra Arn.)

T. Salajová1, J. Salaj1
1 Institute of Plant Genetics, Slovak Academy of Sciences, Nitra, Czechoslovakia

Embryogenic callus was initiated from immature zygotic embryos of black pine on medium DCR supplemented with 2 mg 1-1 2,4-D and 0.5 mg 1-1 BAP. The diploid number of chromosomes confirmed the origin of callus from zygotic embryos. The callus was white, glossy, mucilaginous and contained somatic embryos consisted of an embryonic region with densely cytoplasmic cells and suspensor region with long vacuolated cells. Although somatic embryos with green cotyledons were recognisable after ABA treatment and subsequent transfer to growth-regulator free media whole plants have not yet been obtained.

Received: May 30, 1991; Accepted: January 6, 1992; Published: May 1, 1992  Show citation

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Salajová, T., & Salaj, J. (1992). Somatic embryogenesis in European black pine (Pinus nigra Arn.). Biologia plantarum, 34(3-4), 213-218. doi: 10.1007/BF02925871
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