biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 34:367, 1992 | DOI: 10.1007/BF02923581

Phosphatidylinositol 4,5-bisphosphate specific phospholipase C inPharbitis nil membranes

P. L. R. Bonner1, S. L. Prior1, A. M. Hetherington2, P. J. Lumsden1
1 Department of Applied Biology, University of Central Lancashire, Preston, UK
2 Institute of Environmental and Biological Sciences, Lancaster University, Lancaster, UK

Phosphatidylinositol 4,5-bisphosphate specific phospholipase C has been detected in a membrane preparation fromPharbitis nil cotyledons. The enzyme has a pH optimum of 6.8 and activated by calcium ions, deoxycholate, phosphatidylinositol and phosphatidylethanolamine. The enzyme is inhibited to varying degrees by Tween 20, Triton XI00, zinc, copper, cobalt and manganese ions and phosphatidylserine. G-protein activators do not affect the activity ofPharbitis nil phospholipase C. Analysis of the products of the reaction by HPLC shows inositol 1,4,5-trisphosphate from phospholipase C and inositol bisphosphate from inositol-1 and -5 phosphatase activity.

Received: July 11, 1991; Accepted: August 22, 1991; Published: September 1, 1992  Show citation

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Bonner, P.L.R., Prior, S.L., Hetherington, A.M., & Lumsden, P.J. (1992). Phosphatidylinositol 4,5-bisphosphate specific phospholipase C inPharbitis nil membranes. Biologia plantarum, 34(5-6), 367. doi: 10.1007/BF02923581
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