Biologia plantarum 35:103-105, 1993 | DOI: 10.1007/BF02921129
Effect of the storage conditions on the activity of the potato virus A in ELISA
- 1 Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Praha 6, Czech Republic
We followed the effect of storage conditions on the serological activity of the potato virus A (PVA) in the ELISA test. Storage of the purified PVA in 0.05 M borate buffer, pH 8.3 at -70°C represents the optimum conditions. The activity decreased 40% in comparison to the original activity after four months. Lyophilization of infected leaves or the leaf homogenate in the extraction buffer were found to be the best methods for the storage of the PVA in natural conditions. After 12 months, the activity of PVA decreased to 80% and 50% of the original activity in the case of lyophilized leaves and leaf homogenates, respectively.
Received: September 18, 1992; Accepted: December 9, 1992; Published: March 1, 1993 Show citation
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