Biologia plantarum 35:121-124, 1993 | DOI: 10.1007/BF02921134
Restriction site polymorphism in rDNA repeat unit ofHordeum vulgare for some restriction endonucleases
- 1 Research Institute for Plant Production, Piešťany, Slovakia
Restriction fragment length polymorphism in rDNA repeat unit for eight restriction endonucleases was studied in two genotypes of barley (Hordeum vulgare L.). Both genotypes contained restriction sites for EcoRI, EcoRV, PvuII and Sau3239I in rDNA repeat unit and contained two different rDNA repeat unit length variants 9.7 kbp and 9.1 kbp.
Received: November 28, 1991; Accepted: April 8, 1992; Published: March 1, 1993 Show citation
References
- Ananiev, E.V., Bochkanov, S.S., Ryzhic, M.V., Sonina, N.V., Chernyshev, A.I., Shchypkova, N.I., Yakovleva, E.Yu.: [Characterization of the cloned repeated DNA sequences from the barley genome.]-Genetika22: 920-928, 1986. [In Russ.]
- Dellaporta, S.L., Wood, J., Hicks, J.B.: A plant DNA minipreparation: Version II.-Plant mol. Biol. Rep.1: 19-21, 1983.
Go to original source... - Flavell, R.B.: The structure and control of expression of ribosomal RNA genes.-Oxford Surv. Plant mol. Cell Biol.3: 252-274, 1986.
- Flavell, R.B.: Variation in structure and expression of ribosomal DNA loci in wheat.-Plant mol. Genet.31: 963-968, 1989.
Go to original source... - Hemleben, V., Ganal, M., Gerstner, J., Schiebel, K., Torres, R.A.: Organization and length heterogeneity of plant ribosomal RNA genes.-In: Kahl, G. (ed.): Architecture of Eucaryotic Genes. Pp. 371-383. VCH, Heidelberg 1988.
- Kato, A., Nakajima, T., Yamashita, J., Yakura, K., Tanifuji, S.: The structure of the large spacer region of the rDNA inVicia faba andPisum sativum.-Plant mol. Biol.14: 983-993, 1990.
Go to original source... - Molnar, S.J., Fedak, G.: Polymorphism in ribosomal DNA repeat units of 12Hordeum species.- Genome32: 1124-1127, 1989.
Go to original source... - Molnar, S.J., Gupta, P.K., Fedak, G., Wheatcroft, R.: Ribosomal DNA repeat unit polymorphism in 25Hordeum species.-Theor. appl. Genet.78: 387-392, 1989.
Go to original source... - Rogers, S.O., Bendich, A.J.: Ribosomal RNA genes in plants: variability in copy number and in the intergenic spaces.-Plant. mol. Biol.9: 509-520, 1987.
Go to original source... - Saghai-Maroof, M.A., Soliman, K.M., Jorgensen, R.A., Allard, R.W.: Ribosomal RNA spacer-lenght polymorphism in barley: Mendellian inheritance, chromosomal location, and population dynamics.-Proc. nat. Acad. Sci. USA81: 8014-8018, 1984.
Go to original source... - Sambrook, J., Fritsch, E.F., Maniatis, T.: Molecular cloning. A Laboratory Manual. 2nd Ed., Cold Spring Harbor Laboratory Press, New York 1989.



