biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 35:147-150, 1993 | DOI: 10.1007/BF02921140

Effect of ATP concentration and temperature on firefly luciferase activity

I. Gális1, J. Jirásková1
1 Institute of Plant Molecular Biology, Academy of Sciences of the Czech Republic, Èeské Budìjovice, Czech Republic

The dependence of luciferase activity in the homogenate of leaves of transgenic tobacco plants with chimeric firefly luciferase gene on ATP concentration and temperature was studied. The optimum ATP concentration was between 0.625 mM and 2.5 mM. The activity rapidly decreased if the homogenate was kept in 25°C and is completely lost during 30 min.

Received: November 28, 1991; Accepted: February 10, 1992; Published: March 1, 1993  Show citation

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Gális, I., & Jirásková, J. (1993). Effect of ATP concentration and temperature on firefly luciferase activity. Biologia plantarum, 35(1), 147-150. doi: 10.1007/BF02921140
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