biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 35:161, 1993 | DOI: 10.1007/BF02925932

ATP-and NADH-dependent membrane potential generation in plasmalemma enriched vesicles from parenchyma of dormant and non-dormant jerusalem artichoke tubers

G. Pétel1, M. Gendraud1
1 Laboratoire de Physiologie el Génétique Végétales, Université Blaise Pascal, CEDEX 0l, France

Using a membrane potential probe, Oxonol VI, it was possible to demonstrate generation of ATP- and NADH-dependent membrane potential across the plasmalemma, with membrane vesicles derived from parenchyma cells of Jerusalem artichoke tubers(Helianthus tuberosus L.). It was shown that ATP- and NADH-dependent membrane potential generation was higher in dormant material than in non-dormant tissue and that the effects of ATP and NADH on membrane potential generation were additive. ATP-dependent potential generation was sensitive to vanadate, an inhibitor of plasmalemma ATPase activity. The results are discussed in relation to the properties of the different enzymes bound to the plasma membrane, the morphogenetic potentialities of tuber buds and the hypothesis that tuber dormancy could be an extreme case of nutrient deficiency induced by short-distance intercellular relationships.

Received: August 24, 1992; Accepted: December 8, 1992; Published: June 1, 1993  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Pétel, G., & Gendraud, M. (1993). ATP-and NADH-dependent membrane potential generation in plasmalemma enriched vesicles from parenchyma of dormant and non-dormant jerusalem artichoke tubers. Biologia plantarum, 35(2), 161. doi: 10.1007/BF02925932
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Belkoura, M., Marigo, G.: The proton-pump at the plasmalemma ofCatharanthus roseus cells. Biochimie68: 1299-1302, 1986. Go to original source...
    2. Bown, A.W., Crawford, L.A.: Evidence that H+ efflux stimulated by redox activity is independent of plasma membrane ATPase activity. Physiol. Plant.73: 170-174, 1988. Go to original source...
    3. Courduroux, J.C.: Etude du mécanisme physiologique de la tubérisation chez le Topinambour(Helianthus tuberosus L.). Ann. Sci. Nat. Bot.8: 215-356, 1967.
    4. Delrot, S., Bonnemain, J.L.: Involvement of protons as substrate for the sucrose carrier during phloem loading inVicia faba leaves. Plant Physiol.67: 560-564, 1981. Go to original source...
    5. Felle, H., Bentrup, F.W.: Hexose transport and membrane depolarization inRiccia fluitans. Planta147: 471-476, 1980. Go to original source...
    6. Fol, M., Frachisse, J.M., Pétel, G., Gendraud, M.: Effect of cyanide on the membrane potential of Jerusalem artichoke (Helianthus tuberosus L.) tuber parenchyma: different responses in relation to dormancy. Compt. rend. Acad. Sci. Paris, Sér.III309: 551-556, 1989.
    7. Gendraud, M., Lafleuriel, J.: Characteristics of sucrose and tetraphenylphosphonium uptake by parenchyma ofin vitro grown dormant and non dormant Jerusalem artichoke tubers. Physiol. vég.21: 1125-1133, 1983.
    8. Hassidim, M., Rubinstein, B., Lerner, H.R., Reinhold, L.: Generation of a membrane potential by electron transport in plasmalemma-enriched vesicles of cotton and radish. Plant Physiol.85: 872-875, 1987. Go to original source...
    9. Larsson, C, Widell, S., Sommarin, M.: Inside-out plasma membrane vesicles of high purity obtained by aqueous two-phase partitioning. FEBS Lett.229: 289-292, 1988. Go to original source...
    10. Macri, F., Vianelo, A., Cocucci, M.C.: Mycochrome decreases ATP-driven proton translocation in plant plasma membrane- and tonoplast-enriched vesicles. Plant Cell Environ.10: 369-373, 1987. Go to original source...
    11. Pétel, G., Gendraud, M.: Contribution to the study of ATPase activity in plasmalemma-enriched fractions from Jerusalem artichoke tubers (Helianthus tuberosus L.) in relation to their morphogenetic properties. J. Plant Physiol.123: 373-380, 1986. Go to original source...
    12. Pétel, G., Gendraud, M.: Activité d'un système oxydoréducteur au niveau du plasmalemme des cellules de parenchyme de Topinambour (Helianlhus tuberosus L.) en relation avec les propriétés morphogénétiques des tubercules. Compt. rend. Acad. Sci. Paris, Sér.III305: 51-54, 1987.
    13. Pétel, G., Gendraud, M: Localization in sucrose gradients of pyrophosphatase activities of Jerusalem artichoke (Helianlhus tuberosus L.) tubers microsomal fractions. J. Plant Physiol.134: 466-470, 1989. Go to original source...
    14. Pétel, G., Lafleuriel, J., Dauphin, G., Gendraud, M.: Cytoplasmic pH and plasmalemma ATPase activity of parenchyma cells during the release of dormancy of Jerusalem artichoke tubers. Plant Physiol. Biochem.30: 379-382, 1992a.
    15. Pétel, G., Sueldo, R., Coudret, A., Gendraud, M.: Plasmalemma fluidity in parenchyma cells from Jerusalem artichoke (Helianlhus tuberosus L.) tubers during the break of dormancy. Biol. Plant.34: 373-380, 1992b. Go to original source...
    16. Rubinstein, B., Stern, A.I.: Relationship of transplasmalemma redox activity to proton and solute transport by roots ofZea mays. Plant Physiol.80: 805-811, 1986. Go to original source...
    17. Rubinstein, B., Stern, A.I., Stout, R.G.: Redox activity at the surface of oat root cells. Plant Physiol.76:386-391, 1984. Go to original source...
    18. Singh, S.P., Sudershan Kesav, B.V., Briskin, DP.: Reconstitution and rapid partial purification of the red beet plasma membrane ATPase. Physiol. Plant.69: 617-626, 1987. Go to original source...

    Return to the content