biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 35:179, 1993 | DOI: 10.1007/BF02925936

Some factors affecting somatic embryogenesis efficiency in soybean (Glycine max (L.) Merr.)

M. Griga1
1 Research Institute of Technical Crops and Legumes, Oseva, ©umperk, Czech Republic

Selected factors affecting somatic embryogenesis efficiency have been studied, namely genotype, explant type and its orientation in the medium, different basal media, different auxins for somatic embryo induction, and two ways of donor plant cultivation. The key role is played by genotype and auxin used, the minimum effect was observed due to basal media. In the series of subsequent experiments we have found the best combination of individual factors as follows: cv. Altona, 10 uM 2,4-D, L2 basal medium, central part of immature cotyledon as initial expiant oriented by adaxial side down on the agar medium, and field grown donor plants. This combination exhibited 100 % embryogenic explants with 5.43 ± 0.65 somatic embryos per expiant,i.e. somatic embryogenesis efficiency 5.43.

Received: May 14, 1992; Accepted: November 23, 1992; Published: June 1, 1993  Show citation

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Griga, M. (1993). Some factors affecting somatic embryogenesis efficiency in soybean (Glycine max (L.) Merr.). Biologia plantarum, 35(2), 179. doi: 10.1007/BF02925936
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