biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 35:491, 1993 | DOI: 10.1007/BF02928021

In vitro modification of spinach plasmalemma thickness

P. Crespi1, M. Crèvecoeur1, C. Penel1, H. Greppin1
1 Laboratory of Plant Biochemistry and Physiology, 3, Place de I'Université, Genéve 4, Switzerland

Floral induction in the long day plant spinach (Spinacia oleracea) has been shown to be accompanied by a thickening of plasmalemma. This change was observed at early evocation, in both shoot apices and leaves, as well as after inducing GA3 treatment. To get further information on this thickening, plasma membranes from spinach leaves were isolated, in the present study, using aqueous two phase partitioning and the effect of variousin vitro treatments on their thickness was investigated. The average plasmalemma thickness was unaffected by Na+ and K+ ions. It was increased upon the effect of either Ca2+ or gibberellic acid. A thickening of plasmalemma was also observed when plasma membranes from vegetative plants were incubated with a cytosolic preparation from photoinduced plants. The results were discussed in relation with the plasmalemma modifications previously reported in spinach.

Received: June 3, 1992; Accepted: September 21, 1992; Published: December 1, 1993  Show citation

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Crespi, P., Crèvecoeur, M., Penel, C., & Greppin, H. (1993). In vitro modification of spinach plasmalemma thickness. Biologia plantarum, 35(4), 491. doi: 10.1007/BF02928021
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