biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 35:499-504, 1993 | DOI: 10.1007/BF02928023

Plant regeneration through somatic embryogenesis in peanut (Arachis hypogaea L.)

S. Eapen1, L. George1, P. S. Rao1
1 Bhabha Atomic Research Centre, Trombay, Bombay, India

Somatic embryos were induced from immature cotyledons and immature embryonal axis ofArachis hypogaea L. on L-6 basal medium supplemented with NAA, picloram or 2,4-D at 5-50 mg 1-1. Immature embryonal axis produced a higher number of somatic embryos in comparison with immature cotyledons. The highest number of responding cultures was produced on medium supplemented with NAA (50 mg 1-1), while the highest average number of somatic embryos per culture was produced on medium with 2,4-D (10 or 20 mg 1-1) and picloram (30 mg 1-1) from cotyledons. The somatic embryos developed into plants on basal medium supplemented with activated charcoal and about 100 plants were successfully transferred to the field.

Received: June 26, 1992; Accepted: November 5, 1992; Published: December 1, 1993  Show citation

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Eapen, S., George, L., & Rao, P.S. (1993). Plant regeneration through somatic embryogenesis in peanut (Arachis hypogaea L.). Biologia plantarum, 35(4), 499-504. doi: 10.1007/BF02928023
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