biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 37:185, 1995 | DOI: 10.1007/BF02913208

Identification and relationships of cultivated accessions fromLolium-Festuca complex based on RAPD fingerprinting

I. Wiesner1, P. Samec1, V. Našinec1
1 The Institute of Plant Molecular Biology, Czech Academy of Sciences, České Budějovice, Czech Republic

Randomly Amplified Polymorphic DNA (RAPD) technique with 15 arbitrary primers was used to identify and reveal relationships of the accessions comprising 4 species ofLolium-Festuca complex. Altogether 252 RAPD markers were considered in statistical treatment, 60 of which could be identified as potentially taxon-specific. All analyzed taxa were fully distinguishable using RAPD markers.Lolium-Festuca relationships based on RAPD data were evaluated using cluster analysis (UPGMA) and principle coordinate analysis (PCO). The results of UPGMA as well as PCO performed on data pooled from all RAPD profiles support separation of the two generaLolium andFestuca.

Keywords: cluster analysis; fescue; molecular markers; Poaceae; principal coordinate analysis; ryegrass

Received: June 22, 1994; Accepted: November 15, 1994; Published: June 1, 1995  Show citation

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Wiesner, I., Samec, P., & Našinec, V. (1995). Identification and relationships of cultivated accessions fromLolium-Festuca complex based on RAPD fingerprinting. Biologia plantarum, 37(2), 185. doi: 10.1007/BF02913208
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