biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 37:205, 1995 | DOI: 10.1007/BF02913212

Somatic embryogenesis and plant regeneration from shoot-tip explants inPhoenix dactylifera L.

I. El Hadrami1, R. Cheikh2, M. Baaziz1
1 Laboratoires de Physiologie Végétale et de Biochimie et Biologie Moleculaire des Plantes, Faculté des Sciences-Semlalia, Marrakech, Morocco
2 Laboratoire de Physiologie Végétale, INRA-Marrakech, Morocco

For maximum avoidance of somaclonal variation risks, the commonly used medium for somatic embryogenesis inPhoenix dactylifera has been lowered in growth regulators and activated charcoal. When initially cultured on MS basal medium containing only 150 mg dm-3 charcoal, 5 mg dm-3 2,4-dichlorophenoxyacetic acid (2,4-D) and 5 mg dm-3 benzylaminopurine (BAP), 10 to 20% of shoot-tip explants developed into embryogenic calli. The embryogenic potential has been maintained for over 24 months with no decline. In addition, this medium has been found to be more efficient than conventionaly one containing 3 g dm-3 charcoal, 100 mg dm-3 2,4-D and 3 mg dm-3 2-isopentyladenosine (2IP). Plantlet regeneration was achieved when somatic embryos were subcultured to medium with 0.1 mg dm-3 2,4-D and 0.5 mg dm-3 BAP or without growth regulators.

Keywords: benzylaminopurine; charcoal; date palm; 2,4-dichlorophenoxylacetic acid; 2-isopentyladenosine; tissue browning

Received: April 18, 1994; Accepted: October 27, 1994; Published: June 1, 1995  Show citation

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El Hadrami, I., Cheikh, R., & Baaziz, M. (1995). Somatic embryogenesis and plant regeneration from shoot-tip explants inPhoenix dactylifera L. Biologia plantarum, 37(2), 205. doi: 10.1007/BF02913212
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