biologia plantarum

Biologia plantarum 37:273-283, 1995 | DOI: 10.1007/BF02913226

Salinity-induced changes in the structure and ultrastructure of bean root cells

P. Cachorro¹, E. Olmos¹, A. Ortiz², A. Cerdá¹

¹ Centro de Edafología y Biología Aplicada del Segura, CSIC, Murcia, Spain

² Facultad de Veterinaria, Universidad de Murcia, Murcia, Spain

The effect of 80 mM NaCl on the structure and ultrastructure of root cells ofPhaseolus vulgaris plants has been investigated. Roots of plants treated with NaCl were shorter and had less secondary roots than control plants. In control plants, epidermal cells were isodiametric and uniformly placed forming a thin layer, whereas in stressed plants, the shape and disposition of epidermal cells was less regular. The cortical cells of control plant were round-shaped and distributed allowing large and well defined intercellular spaces, whereas stressed plants presented irregular cells, which were interdigitated, thus decreasing the volume of the intercellular spaces. Presence of 80 mM NaCl did not result in significant differences in the number, shape or distribution of the cell organelles. Membrane vesiculation was often observed in cells of NaCl treated plants. Addition of 80 mM NaCl to the growth medium considerably increased the leakage of solutes from intact plant roots back to the solution especially K⁺ and Ca²⁺.

Keywords: cortical cells; epidermal cells; intercellular spaces; leakage; membrane vesiculation; NaCl; Phaseolus vulgaris

Received: August 1, 1994; Accepted: October 17, 1994; Published: June 1, 1995  Show citation