biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 37:329-334, 1995 | DOI: 10.1007/BF02913236

Overcoming prefertilization barriers in the crossDiplotaxis siettiana ×Brassica juncea using irradiated mentor pollen

B. K. Sarmah1, N. Sarla1
1 Division of Genetics, Indian Agricultural Research Institute, New Delhi, India

Hybridization between the nearly extinct speciesDiplotaxis siettiana andBrassica juncea is prevented because of strong prefertilization barriers. Use of mentor pollen ofD. siettiana irradiated with 1000 Gy gamma radiation before the incompatible pollination led to fertilization. 5 d after pollination 17% ovules showed entry of pollen tubes, 10 d after pollination 27% ovules showed small globular embryos which grew no further. No embryos were found in control pollinations. Thus , use of irradiated mentor pollen brings about fertilization in this difficult cross and hybrids can be obtained if embryos are rescued.

Keywords: gamma radiation; incompatibility; pollen germination

Received: October 15, 1993; Accepted: June 14, 1994; Published: June 1, 1995  Show citation

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Sarmah, B.K., & Sarla, N. (1995). Overcoming prefertilization barriers in the crossDiplotaxis siettiana ×Brassica juncea using irradiated mentor pollen. Biologia plantarum, 37(2), 329-334. doi: 10.1007/BF02913236
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