biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 37:321-327, 1995 | DOI: 10.1007/BF02913972

Detection of DNA polymorphism among pea cultivars using RAPD technique

P. Samec1, V. Našinec1
1 Institute of Plant Molecular Biology, Academy of Sciences of the Czech Republic, České Budějovice, Czech Republic

An influence of some Random Amplified Polymorphic DNA (RAPD) reaction factors on resulting banding pattern and the ability of RAPD technique to detect DNA polymorphism among six economically important pea cultivars was tested. Relatively high level of DNA polymorphism among peas was observed, using polyacrylamide/urea gels and silver staining. Altogether 13 arbitrarily designed primers produced 313 amplification products. In addition 59 polymorphisms were found. These polymorphisms can serve as potential genetic markers. RAPD data were processed using cluster analysis and plotted as dendrogram. Each tested cultivar was clearly distinguished from the others. Moreover,Pisum sativum andP. sativum subsp.arvense cultivars were separated into 2 different clusters, according to their systematic relationships.

Keywords: arbitrary primers; molecular markers; Pisum sativum

Received: January 20, 1995; Accepted: March 23, 1995; Published: September 1, 1995  Show citation

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Samec, P., & Našinec, V. (1995). Detection of DNA polymorphism among pea cultivars using RAPD technique. Biologia plantarum, 37(3), 321-327. doi: 10.1007/BF02913972
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