biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 37:335-340, 1995 | DOI: 10.1007/BF02913975

In vitro propagation ofBoswellia serrata Roxb

S. D. Purohit1, K. Tak1, G. Kukda1
1 Plant Biotechnology Laboratory, Department of Botany, M.L. Sukhadia University, Udaipur, India

Anin vitro procedure for large scale multiplication ofBoswellia serrata Roxb. has been developed using cotyledonary node segments. In average 4 shoots per node were obtained on Murashige and Skoog's (MS) medium containing 0.5 mg dm-3 6-benzylaminopurine (BAP) and 0.05 mg dm-3 napthaleneacetic acid (NAA) within 22 d. By repeated subculture technique 90-100 shoots per node could be obtained after 88 d of initial culture. Shoots could be rooted on MS medium containing 1/4 salts, 1% saccharose, and a combination of 0.5 mg dm-3 indole-3-butyric acid (IBA) and 0.25 mg dm-3 indole-3-acetic acid (IAA). Addition of antioxidants like polyvinylpyrrolidone (PVP-50 mg dm-3) and ascorbic acid (100 mg dm-3) in both multiplication and rooting media prevented browning of cultures. Approximately 80% of shoots rooted within 8-10 d. Rooted plantlets were kept for 15 d in culture bottles containing SoilriteTM irrigated with a nutrient solution containing 1/4 MS salts and finally transferred to pots containing soil: SoilriteTM (1∶1), mixture with 70% transplantation success.

Keywords: afforestation; benzylaminopurine; indole-3-acetic acid; indole-3-butyric acid; kinetin; napthaleneacetic acid

Received: September 20, 1994; Accepted: March 23, 1995; Published: September 1, 1995  Show citation

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Purohit, S.D., Tak, K., & Kukda, G. (1995). In vitro propagation ofBoswellia serrata Roxb. Biologia plantarum, 37(3), 335-340. doi: 10.1007/BF02913975
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    References

    1. Aitken-Christie, J., Connett, M.: Micropropagation of forest trees.-In: Kurata, K., Kozai, T. (ed.): Transplant Production Systems. Pp. 163-194. Kluwer Academic Publishers, Dordrecht-Boston-London 1992. Go to original source...
    2. Hu, C.Y., Wang, P.J., Meristem, shoot tip and bud cultures.-In: Evans, D.A., Sharp, R.W., Ammirato, P.V., Yamada, Y. (ed.): Handbook of Plant Cell Culture. Vol. 1. Pp. 177-227. Macmillan, New York 1983.
    3. Joshi, R.:In vitro studies on forest plants of Rajasthan.-Ph.D. Thesis, University of Jodhpur, Jodhpur 1991.
    4. Mascarenhas, A.F., Muralidharan, E.M.: Tissue culture of forest trees in India.-Curr. Sci.58: 606-613, 1989.
    5. McComb, J.A., Newton, S.: Propagation of Kangaroo Paws using tissue culture.-J. hort. Sci.56: 181-183, 1981. Go to original source...
    6. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures.-Physiol. Plant.15: 473-497, 1962. Go to original source...
    7. Rathore, T.S., Singh, R.P., Shekhawat, N.S.: Clonal propagation of desert teak (Tecomella undulata) through tissue culture.-Plant Sci.79: 217-222, 1991. Go to original source...
    8. Rathore, T.S., Deora, N.S., Shekhawat, N.S.: Cloning ofMaytenus emarginata (Willd.) Ding Hou-a tree of the Indian Desert, through tissue culture.-Plant Cell Rep.11: 449-451, 1992. Go to original source...
    9. Sharma, S.: A census of rare and endemic flora of South-East Rajasthan.-In: Jain, S.K., Rao, R.R. (ed.): Threatened Plants of India. Pp. 630-670. Botanical Survey of India, Howrad 1983.

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