biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 37:381, 1995 | DOI: 10.1007/BF02913984

Kinetics of phosphate uptake in excised maize root

P. Kumar1, U. S. Mishra2, R. C. Pant1
1 Department of Plant Physiology, G.B. Pant University of Agriculture and Technology, Pantnagar, India
2 Department of Plant Physiology, Khandwa Agriculture College, Khandwa, India

The short term uptake of phosphate involving 10 min absorption followed by 5 min desorption, both at 30 °C, in the concentration range 1.0×10-9 to 7.5×10-2 M KH2PO4 by fresh and washed maize (Zea mays L. cv. Ganga Safed-2) roots can be described by a single isotherm having five phases (0 and I-IV) with regularly spaced kinetic constants. Almost identical kinetics were observed in both fresh and washed maize roots. The kinetics of phase 0 in the concentration range 1.0×10-9-3.0×10-5 M. was sigmoidal in fresh maize roots, however, in washed tissue exhibited 2 phases termed here as 0a and 0b. 0a covered the concentration range 1.0×10-9-5.0×10-6 M and 0b 6.0×10-6-3.0×10-5 M. In the concentration range 1.0×10-4-7.5×10-2 M four distinct phases, termed as I, II, III and IV were evident in both fresh and washed maize roots. Each phase obeyed Michaelis-Menten kinetics. The values of Km and Vmax have been estimated for each phase. The uptake isotherm was accompanied by discontinuous transitions.

Keywords: kinetic constants; Michaelis-Menten kinetics; single isotherm; Zea mays

Received: June 15, 1994; Accepted: December 5, 1994; Published: September 1, 1995  Show citation

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Kumar, P., Mishra, U.S., & Pant, R.C. (1995). Kinetics of phosphate uptake in excised maize root. Biologia plantarum, 37(3), 381. doi: 10.1007/BF02913984
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