biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 37:467, 1995 | DOI: 10.1007/BF02914001

Improvement ofArabidopsis thaliana seed transformation efficiency

D. Pavingerová1, M. Ondřej1
1 Institute of Plant Molecular Biology, Academy of Sciences of Czech Republic, České Budějovice, Czech Republic

Agrobacterium tumefaciens induced transgenosis by treatment of germinatingArabidopsis thaliana seed embryos has been achieved with differentAgrobacterium strains including the strain LBA4404, which was ineffective in seed transformation experiments of the other authors. The frequency of transgenosis was increased several times by application of acetosyringone to the growingA. tumefaciens suspension cultures. The DNA demethylating agent 5-azacytidine partly restored the distorted Mendelian segregation ratios in the offspring of transgenic plants.

Keywords: acetosyringone; Agrobacterium tumefaciens; 5-azacytidine

Received: February 3, 1995; Accepted: May 5, 1995; Published: September 1, 1995  Show citation

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Pavingerová, D., & Ondřej, M. (1995). Improvement ofArabidopsis thaliana seed transformation efficiency. Biologia plantarum, 37(3), 467. doi: 10.1007/BF02914001
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