biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 38:27, 1996 | DOI: 10.1007/BF02879628

Tissue culture and transformation ofOenothera biennis

D. Pavingerová1, I. Gális1, M. Ondřej1
1 Institute of Plant Molecular Biology, Academy of Sciences of the Czech Republic, české Budějovice, Czech Republic

Five cultivars ofOenothera biennis have been tested for callogenesis and organogenesis on different media. The cultivar CV3 has been transformed byAgrobacterium tumefaciens strain which introduces into the plant genome kanamycin resistance gene and the T-DNAipt gene which causes increased levels of cytokinins. Transformed tissues showed elevated levels of cytokinins and grew as teratomas forming clumps of short, branched shoots with small modified leaves. Roots appeared rarely in later subcultivations of some teratomous clones.

Keywords: Agrobacterium tumefaciens; cytokinins; regeneration; regeneration

Received: August 29, 1995; Accepted: October 3, 1995; Published: March 1, 1996  Show citation

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Pavingerová, D., Gális, I., & Ondřej, M. (1996). Tissue culture and transformation ofOenothera biennis. Biologia plantarum, 38(1), 27. doi: 10.1007/BF02879628
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