biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 38:377, 1996 | DOI: 10.1007/BF02896666

Catalase activity in developing seedling of opium poppyPapaver somniferum L

L. Bezáková1, M. Mikuš2, H. Šmorgovičova1, P. Kovács1, M. Pšenák1
1 Department of Cell and Molecular Biology of Drugs, Faculty of Pharmacy, Comenius University, Bratislava, Slovak Republic
2 Department of Plant Physiology and Biotechnology, Comenius University, Bratislava, Slovak Republic

Catalase is an enzyme unique to glyoxysomes in developing poppy seedlings. Catalase activity is very low in endosperm and in embryo of germinating poppy seeds. During postgerminative growth and development the enzyme activity increases rapidly with maximum in endosperm on day 2 and in developing seedling on day 3. A rapid decline of enzyme activity parallells the extension growth of poppy seedlings. Three electrophoretic forms of catalase have been detected in isolated glyoxysomes and partially purified catalase preparation. Electron microscopic observation indicates the presence of catalase in glyoxysomes of parenchyma cells of poppy seedling cotyledons. Numerous lipid bodies and electron-dense deposits in vacuoles are the most characteristic feature of these cells.

Keywords: cotyledons; endosperm; glyoxysomes; Sacharose gradient; seedlings

Received: May 2, 1995; Accepted: July 18, 1995; Published: September 1, 1996  Show citation

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Bezáková, L., Mikuš, M., Šmorgovičova, H., Kovács, P., & Pšenák, M. (1996). Catalase activity in developing seedling of opium poppyPapaver somniferum L. Biologia plantarum, 38(3), 377. doi: 10.1007/BF02896666
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