biologia plantarum

Biologia plantarum 38:571, 1996 | DOI: 10.1007/BF02890609

Distribution of the stress-related anionic peroxidases in different cucumber organs

V. Repka¹, I. Fischerová¹

¹ Laboratory of Molecular Biology and Virology, Complex Research Institute of Viticulture and Enology, Bratislava

The distribution of the stress-related anionic peroxidase (srPRX) activity was investigated in various organs of cucumber (Cucumis sativus L. cv. Laura) during their development by activity staining and immunoblotting. In shoots, including cotyledon, leaf, stem and tendril, three stress-related peroxidase isoenzymes were present, particularly in old ones. The PRX1 was the only srPRX isoenzyme found in both young and old roots. As fruits became mature, srPRX activity increased dramatically and was particularly enriched in the external parts of the fruit. The PRX1 isoenzyme was highly accumulated in the course of seed germination, while the absence of other two srPRX isoenzymes (PRX2 and PRX3) was recorded. The possible function of the srPRX is discussed, with respect to this spatio-temporal distribution.

Keywords: Cucumis; electrophoresis; fruits; immunological study; seed germination; tobacco necrosis virus; vegetative tissues kgWestern blotting

Received: February 12, 1996; Accepted: May 9, 1996; Published: December 1, 1996  Show citation