biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 39:43-53, 1997 | DOI: 10.1023/A:1000940400276

Polymerase chain reaction detect polymorphisms and trait association in soybean

K. Zhang1, K.M. Soliman1, V.T. Sapra1
1 Department of Plant and Soil Science, Alabama A&M University, Normal, USA

Five sets of synthetic oligonucleotide (20-to 24-mers containing no intenal repeats) primers of known gene sequences [yellow lupin nodule specific (hydroxyl) proline-rich protein, pearl millet alcohol dehydrogenase, Pisum sativum heat shock proteins, Drosophila homeobox, and tRNA] were used to differentiate 73 soybean accessions, including 56 Glycine max (L.) Merr. and 17 G. soja Zucc. & Sieb. The amplified genetic markers revealed polymorphic bands for most genotypes studied. The χ2-analyses of the results showed that several fragments produced with these gene primers were associated non-randomly with resistance to Phytophothora, maturity, seed size, flower colour, seed coat colour, seed hilum colour, growth type, and leaf shape. These markers appear to be valuable for differentiation of G. max and G. soja species and genotypes within these species.

Keywords: Glycine max; Glycine soja; homeobox
Subjects: accessions, soybean; genetic markers, soybean genotypes; Glycine max; Glycine soja; polymerase chain reaction, polymorphism; soybean, polymorphism, polymerase

Published: July 1, 1997  Show citation

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Zhang, K., Soliman, K.M., & Sapra, V.T. (1997). Polymerase chain reaction detect polymorphisms and trait association in soybean. Biologia plantarum, 39(1), 43-53. doi: 10.1023/A:1000940400276
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