biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 39:207-213, 1997 | DOI: 10.1023/A:1000384618661

Does cholinesterase participate in the intercellular interactions in pollen-pistil system?

L.V. Kovaleva1, V.V. Roshchina2
1 Institute of Plant Physiology, Russian Academy of Sciences, Moscow, Russia
2 Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Russia

Hydrolysis of acetylthiocholine and butyrylthiocholine has been observed in aqueous extracts from petunia pollen and pistils. The reproductive organs of self-compatible clone showed a higher rate of choline ester hydrolysis than those of self-incompatible clone. The highest rate of acetylthiocholine hydrolysis blocked by the cholinesterase inhibitors (physostigmine and neostigmine) was characteristic for the pollen of self-compatible clone. The incomplete (25 - 40 %) inhibition of hydrolysis in pistil extracts of self-compatible clone suggests the presence of unspecific esterases. The eight-fold lower hydrolysis was observed in the pistils of self-incompatible clone as compared to the pistils of compatible clone; neostigmine completely blocked this low hydrolytic activity. The treatment of flower buds with physostigmine and neostigmine (10-5 - 10-3 M) decreased the seed production by 10 - 20 % in compatible clone. When the surfaces of pistil stigmae were treated with physostigmine and neostigmine (10-5 - 10-3 M) before pollination, the seed formation was inhibited by 95 % after both self- and cross-pollination.

Keywords: acetylthiocholine; butyrylthiocholine; Petunia hybrida L.; self-compatible and self-incompatible clones; neostigmine; physostigmine

Prepublished online: March 1, 1997; Published: September 1, 1997  Show citation

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Kovaleva, L.V., & Roshchina, V.V. (1997). Does cholinesterase participate in the intercellular interactions in pollen-pistil system? Biologia plantarum, 39(2), 207-213. doi: 10.1023/A:1000384618661
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    References

    1. Bednarska, E.: The localization of nonspecific esterase and cholinesterase activity in germinating pollen and pollen tube of Vicia faba L. The effect of actinomycin D and cycloheximide.-Biol. Plant. 34: 229-240, 1992. Go to original source...
    2. Bednarska, E., Tretyn, A.A.: Ultrastructural localization of acetylcholinesterase activity in the stigma of Pharbitis nil.-Cell Biol. int. Rep. 13: 275-281, 1989. Go to original source...
    3. Gorun, V., Proinov, I., Baltescu, V., Balaban, J., Barru, O.: Modified Ellman procedure for assay of cholinesterase in crude enzymatic preparations.-Anal. Biochem. 86: 324-326, 1978. Go to original source...
    4. Kovaleva, L.: Gene expression in flowering and the gametophyte-sporophyte interactions-Flowering Newslett. 19: 38-42, 1995.
    5. Kovaleva, L. V., Komarova, E.N.: [Lectins of petunia pistil and intercellular interactions in pollenpistil system.]-Dokl. Akad. Nauk 339: 286-290, 1994. [In Russ.]
    6. Kovaleva, L. V., Musatova, N. A.: [RNAse activity in petunia reproductive organs in connection with incompatibility mechanism.]-Dokl. Akad. Nauk SSSR 223: 762-765, 1975. [In Russ.]
    7. Kovaleva, L. V., Sergeeva, L.I., Zaltsman, O.: [Peroxidase and reproductive process in petunia.]-Dokl. Akad. Nauk 340: 569-570, 1995. [In Russ.]
    8. Marquardt, P., Vogt, J.: Vorkommen, Eigenschaften und Chemische Konstitution des Cholinergischen Faktoren in Honig.-Arzneimittel Forschung 2: 152-155, 1952.
    9. Roshchina, V.V.: Biomediatory v Rasteniyakh. Atsetilkholin i Biogennye Aminy. [Biomediators in Plants. Acetylcholine and Biogenic Amines.]-Biological Center, Pushchino 1991. [In Russ.]
    10. Roshchina, V.V., Melnikova, E.V., Kovaleva, L.V., Spiridonov, N.A.: [Cholinesterase in pollen grains.]-Dokl. Akad. Nauk 337: 424-427, 1994. [In Russ.]
    11. Roshchina, V.V., Semenova, M.N.: Plant cholinesterases: activity and substrate-inhibitory specificity.-J. evol. Biochem. Physiol. 26: 644-651, 1990.
    12. Semenova, M.N., Roshchina, V.V.: [The occurrence of cholinesterase in anthers of higher plants.]-Fiziol. Rast. 40: 255-259, 1993. [In Russ.]

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