biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 39:251-256, 1997 | DOI: 10.1023/A:1000353206366

Low irradiance stress tolerance in rice (Oryza sativa L.)

M.M. Viji1, M. Thangaraj1, M. Jayapragasam1
1 Centre for Plant Molecular Biology, Tamil Nadu Agricultural University, Coimbatore, India

The effect of low irradiance on three rice cultivars (shade tolerant cvs. Swarnaprabha and CO 43 and shade susceptible cv. IR 20) was studied. The large subunit (LSU) of ribulose-1,5-bisphosphate carboxylase/oxygenase with molecular mass of 55 kDa was reduced in cv. IR 20 grown under low irradiance (LI). Native protein profile studied showed, under LI, reduction in the contents of proteins with RF values 0.03, 0.11 and 0.37. Analysis of chloroplast polypeptides revealed an induction of light-harvesting chlorphyll-protein 2 (LHCP2) under shade. The induction was more expressed in cv. CO 43 than in cv. IR 20. Under LI, in vivo labelled protein bands in the molecular range of 26 - 27 kDa were induced. These proteins were highly turned over in the LI-grown plants of cv. CO 43 than in cv. IR 20. A signal for rbcL gene sequences in EcoRI digested lanes was also found. Isozyme analysis of peroxidase showed an induction of a new band with RF 0.43 in cv. IR 20 subjected to LI.

Keywords: chloroplast proteins; light-harvesting chlorophyll-protein 2; peroxidase; rbcL gene sequences; SDS-PAGE

Prepublished online: March 1, 1997; Published: September 1, 1997  Show citation

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Viji, M.M., Thangaraj, M., & Jayapragasam, M. (1997). Low irradiance stress tolerance in rice (Oryza sativa L.). Biologia plantarum, 39(2), 251-256. doi: 10.1023/A:1000353206366
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