biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 39:285-290, 1997 | DOI: 10.1023/A:1001080906504

Nutrient-encapsulation of potato nodal segments for germplasm exchange and distribution

D. Sarkar1, P.S. Naik1
1 Division of Genetics and Plant Breeding, Central Potato Research Institute, Shimla-, Himachal Pradesh, India

Nutrient-encapsulation technique using in vitro grown nodal segments was developed as an alternative method for distribution of potato germplasm. The nodal cuttings of two potato genotypes, Kufri Jyoti and Kufri Lauvkar, were encapsulated in calcium-free Murashige and Skoog's (MS) medium containing either 2 or 3 % sodium alginate and 0, 1, 2 or 3 % saccharose. The encapsulated segments were stored in tubes with or without semisolid MS medium, and incubated in the dark at 25 ± °C for 3 to 6 weeks. Presence of saccharose in the beads was found detrimental for regrowth of new shoots. In absence of saccharose, about 82 % and 53 % encapsulated segments initiated regrowth after 3 and 6 weeks of dark storage, respectively, in tubes containing MS medium. Storage in empty tubes deteriorated the encapsulated segments, and depressed shoot formation. For potato germplasm distribution, the encapsulated segments can be transported in small tubes containing semisolid MS medium.

Keywords: alginate capsules; microcuttings; sodium alginate; Solanum tuberosum L.
Subjects: alginate capsules, nutrients; callus (tissue) culture, germplasm exchange; cultivar differences, germplasm exchange; germplasm exchange, nutrient encapsulation of nodal segments; nutrient encapsulation of nodal segments; Solanum tuberosum

Published: September 1, 1997  Show citation

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Sarkar, D., & Naik, P.S. (1997). Nutrient-encapsulation of potato nodal segments for germplasm exchange and distribution. Biologia plantarum, 39(2), 285-290. doi: 10.1023/A:1001080906504
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