biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 39:299-302, 1997 | DOI: 10.1023/A:1000688117822

Improved method of total histone isolation from Arabidopsis thaliana

M.R. Przewloka1, A. Jerzmanowski2
1 Laboratory of Plant Molecular Biology, Warsaw University, Poland
2 Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland

Standard methods of isolation of chromatin histones from Arabidopsis thaliana yield strongly degraded proteins when applied to plants grown from seeds in axenic liquid media. For isolation of undegraded histones from Arabidopsis grown in liquid media we used extraction with guanidine hydrochloride followed by selective binding of histones on BioRex 70 resin in the batch system. The quality of obtained proteins is confirmed by SDS polyacrylamide gel electrophoresis.

Keywords: quanidine hydrochloride; SDS PAGE

Prepublished online: March 1, 1997; Published: September 1, 1997  Show citation

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Przewloka, M.R., & Jerzmanowski, A. (1997). Improved method of total histone isolation from Arabidopsis thaliana. Biologia plantarum, 39(2), 299-302. doi: 10.1023/A:1000688117822
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