biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 39:303-308, 1997 | DOI: 10.1023/A:1000692218731

Shoot regeneration and protein synthesis in tomato tissue cultures

A.E. El-Enany1
1 Faculty of Science, Botany Department, Assiut University, Assiut, Egypt

Shoot regenerations from hypocotyls and cotyledons of tomato(Lycopersicon esculentum Mill.) was inhibited by NaCl (100 and 150 mM).Shoot fresh and dry masses were also reduced. Addition of proline (100 mgdm-3) counteracted the inhibitory effect of NaCl. SDS-PAGE analyses of extracted proteins, revealed that in cultures grown in medium with 25 mM NaCl plus proline, extra polypeptides of Mr 190, 58, 45 and 26 kDa accumulated. As NaCl was increased in the medium a new protein of Mr 67 kDa also accumulated.

Keywords: Lycopersicon esculentum; proline; protein accumulation; salinity

Prepublished online: March 1, 1997; Published: September 1, 1997  Show citation

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El-Enany, A.E. (1997). Shoot regeneration and protein synthesis in tomato tissue cultures. Biologia plantarum, 39(2), 303-308. doi: 10.1023/A:1000692218731
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