biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 39:419-425, 1997 | DOI: 10.1023/A:1001088412321

Associations of sugar beet and nitrogen-fixing bacteria in vitro

N. Mrkovački1, S. Mezei1, I. Verešbaranji1, M. Popović2, Z. Sarić1, L. Kovačev1
1 Scientific Institute of Field and Vegetable Crops, Novi Sad, Yugoslavia
2 Faculty of Natural Sciences, Novi Sad, Yugoslavia

The associations of four Azotobacter chroococcum strains (2, 5, 8 14) isolated from the rhizosphere with two sugar beet hybrids (Hy-11 and Dana) grown in vitro have been investigated. All tested strains caused an increase in dry mass of both hybrids almost proportionally to the nitrogen content in the medium. Plant nitrogen content was also higher in inoculated variants. The highest nitrogenase and glutamate dehydrogenase activities were in Hy-11 associated with strain 5 and in hybrid Dana associated with strain 8. These associations were the most effective in N2-fixation 2 weeks after inoculation. The results showed specificity of individual strains with respect to individual hybrids.

Keywords: Azotobacter strains; Beta vulgaris; glutamate dehydrogenase; nitrogenase

Prepublished online: April 1, 1997; Published: November 1, 1997  Show citation

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Mrkovački, N., Mezei, S., Verešbaranji, I., Popović, M., Sarić, Z., & Kovačev, L. (1997). Associations of sugar beet and nitrogen-fixing bacteria in vitro. Biologia plantarum, 39(3), 419-425. doi: 10.1023/A:1001088412321
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    References

    1. Allison, F.F.: Azotobacter inoculation of crops.-J. Histor. Soil Sci. 64: 413-427, 1947. Go to original source...
    2. Berkum, P., Van Bohlool, B.B.: Evaluation of nitrogen-fixation by bacteria in association with roots of tropical grasses.-Microbiol. Rev. 44: 491-517, 1980. Go to original source...
    3. Berkum, P., Van Sloger, P.C.: Interaction of combined nitrogen with the expression of root-associated nitrogenase activity in grasses and with the development of N2 fixation in soybean.-Plant Physiol. 72: 741-745, 1983. Go to original source...
    4. Burns, R.D., Hardy, R.W.F.: Nitrogen fixation in bacteria and higher plants.-Mol. Biol. Biochem. Biophys. 21: 1-189, 1975. Go to original source...
    5. Cooper, R.: Bacterial fertilizers in the Soviet Union.-Soils Fertil. 22: 327-333, 1959.
    6. Farnden, K.J.F., Robertson, J.G.: Methods for studing involved in metabolism related to nitrogenase.-In: Bergersen, F.J. (ed.): Methods for Evaluating Biological Nitrogen Fixation. Pp. 265-314. John Wiley and Sons, Chichester-New York 1980.
    7. Gašić, O., Hong, Z., Popović, M., Lukić, V., Sarić, M., Sarić, Z.: Activity of nitrogenase and nitrogen assimilation enzymes in wheat inoculated with Azotobacter chroococcum.-Biochem. Physiol. Pflanz. 186: 169-178, 1990. Go to original source...
    8. Hardy, R.W.F., Holsten, R.D., Yackson, E.K., Burns, R.C.: The acetylene-ethylene assay for N2 fixation: laboratory and field evaluation.-Plant Physiol. 43: 1185-1207, 1968. Go to original source...
    9. Iswaran, V., Patil, V.D., Sen, A.: Effect of spray of the culture of a bacterium from the phyllosphere of water hyacinth (Eichornia crassyes Mort Solms) on the yield of paddy and wheat.-Plant Soil. 50: 253-255, 1978. Go to original source...
    10. Jagnow, G.: Inoculation of cereal crops and forage grasses with nitrogen-fixing rhizosphere bacteria: possible causes of success and failure with regard to yield response. A review.-Z. Pflanzenernähr. Bodenk. 150: 361-368, 1987. Go to original source...
    11. Khullar, S.S., Chahal, V.P., Kaur, P.P.: Effect of Azotobacter inoculation with Rhizobium and Azotobacter on chlorophyll contents and other characters of carrot, radish, brinjal and chilli.-Indian J. Microbiol. 18: 138-140, 1978.
    12. Krstić, B., Sarić, M., Sarić, Z.: Efficiency of Azotobacter strains depending on nitrogen level and sugar beet genotypes-In: Polsinelli, M., Materassi, R., Vincenzini, M. (ed.): Proceedings of the Fifth International Symposium on Nitrogen Fixation with Non-legumes. Pp. 329-331. Kluwer Academic Publishers, Dordrecht-Boston-London 1991. Go to original source...
    13. Lowry, O.H., Rosenbrough, N.J., Farr, A.L., Randal, R.J.: Protein measurement with the Folin reagent.-J. biol. Chem. 193: 265-275, 1951. Go to original source...
    14. Maskey, S.L.: Effect of inoculation on wheat and paddy.-Nepal J. Agr. 12: 23-33, 1977.
    15. Mrkovački, N., Mezei, S., Kovačev, L.: Specific relationship between Azotobacter strains and sugar beet plants.-Soil Plant 44: 9-17, 1995.
    16. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures.-Physiol. Plant. 15: 473-497, 1962. Go to original source...
    17. Sarić, M.R., Sarić, Z., Govedarica, M.: Specific relations between some strains of diazotrophs and corn hybrid.-Plant Soil 99: 147-162, 1987. Go to original source...
    18. Sarić, M.R., Sarić, Z., Krstić, B.: Specific responses of Azotobacter strains and sugar beet genotypes-In: Polsinelli, M., Materassi, R., Vincenzini, M. (ed.): Proceedings of the Fifth Internationl Symposium on Nitrogen Fixation with Non-legumes. Pp. 333-335. Kluwer Academic Publishers, Dordrecht-Boston-London 1991. Go to original source...
    19. Scarsbrook, C.E.: Nitrogen availability-In: Bartholomew, W.V., Clark, F.E. (ed.): Soil Nitrogen. Pp. 481-502. American Society of Agronomy, Madison 1965. Go to original source...
    20. Viets, F.J.: The plants need for and use of nitrogen-In: Bartholomew, W.W., Clark, F.E. (ed.): Soil Nitrogen. Pp. 503-549. American Society of Agronomy, Madison 1965. Go to original source...
    21. Yahalom, E., Kapulnik, Y., Okon, Y.: Response of Setaria italica to inoculation with Azospirillum brasilense as compared to Azotobacter chroococcum.-Plant Soil 82: 77-85, 1984. Go to original source...

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