biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 39:459-464, 1997 | DOI: 10.1023/A:1001138619224

Biochemical changes induced by accelerated ageing in Bambusa bambos seeds

R. Ravikumar1, G. Ananthakrishnan1, A. Ganapathi1, T. Appasamy1
1 Department of Biotechnology, School of Life Sciences, Bharathidasan University, Tiruchirapalli, Tamil Nadu, India

Decrease in seed viability and germination rate may be caused by biochemical changes associated with seed ageing. Different biochemical assays were conducted to investigate the changes occurring at the ageing of Bambusa bambos seeds. A reduction in the total content of food reserves such as sugars, proteins and lipids were recorded. Decreased activity of peroxidase, acid phosphatase, alkaline phosphatase were also noticed during accelerated ageing. A substantial increase in total free amino acids and the activity of amylases confirms the degradation of stored biomolecules in seeds during ageing.

Keywords: bamboo; α and β-amylase; peroxidase; acid and alkaline phosphatase
Subjects: ageing, accelerated, enzymes; a and b-amylase, seed ageing; bamboo, seed ageing; Bambussa bambos; in vitro culture, shoot-tip culture; medicinal plants, micropropagation; peroxidase, seed ageing; phosphatases, seed ageing; Plantago major

Published: November 1, 1997  Show citation

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Ravikumar, R., Ananthakrishnan, G., Ganapathi, A., & Appasamy, T. (1997). Biochemical changes induced by accelerated ageing in Bambusa bambos seeds. Biologia plantarum, 39(3), 459-464. doi: 10.1023/A:1001138619224
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    References

    1. Abdul-Baki, A.A., Anderson, J.D.: Physiological and biochemical deterioration of seeds.-In: Kozlowski, T. (ed.): Seed Biology, Vol. 2. Pp. 283-315. Academic Press, London 1972. Go to original source...
    2. Basavarajappa, B.S., Shetty, H.S., Prakash, H.S.: Membrane deterioration and other biochemical changes, associated with accelerated ageing of maize seeds.-Seed Sci. Technol. 19: 279-286, 1991.
    3. Baskin, C.C.: Vigor test methods-accelerated ageing.-Assoc. Off. Seed Anal. Newsletter 51(5): 42-52, 1977.
    4. Bernal-Lugo, L., Leopold, A.C.: Changes in soluble carbohydrates during seed storage.-Plant Physiol. 98: 1207-1210, 1992. Go to original source...
    5. Bragdon, J.H.: Estimation of total fat.-J. biol. Chem. 190: 513-514, 1951. Go to original source...
    6. Coolbear, P., Francis, A., Grierson, D.: The effect of low temperature pre-sowing treatment on the germination performance and membrane integrity of artificially aged tomato seeds. J. exp. Bot. 35: 1609-1617, 1984. Go to original source...
    7. Delouche, J.C., Baskin, C.C.: Accelerated aging techniques for predicting the relative storability of seed lots.-Seed Sci. Technol. 1: 427-452, 1973.
    8. Dubois, M., Gillies, K.N., Hamilton, J.K., Rebers, P.A., Smith, F.: Colorimetric method for determination of sugars and related substances.-Anal. Chem. 28: 300-356, 1956. Go to original source...
    9. Dure, L.S.: Site of origin and extend of activity of amylases in maize germination.-Plant Physiol. 35: 925-934, 1960. Go to original source...
    10. Francis, A., Coolbear, P.: Changes in the membrane phospholipid composition of tomato seeds accompanying loss of germination capacity caused by controlled deterioration.-J. exp. Bot. 35: 1764-1771, 1984. Go to original source...
    11. Gidrol, X., Serghini, H., Noubhani, A., Mocquot, B., Mazliak, P.: Biochemical changes induced by accelerated aging in sunflower seeds. I. Lipid peroxidation and membrane damage.-Physiol. Plant. 76: 591-597, 1989. Go to original source...
    12. Harrington, J.F.: Seed storage and longevity.-In: Kozlowski, T. (ed.): Seed Biology, Vol. 3. Pp. 145-245. Academic Press, London 1972. Go to original source...
    13. Harman, G.E., Mattick, R.L.: Association of lipid oxidation with seed aging and death.-Nature 260: 323-324, 1976. Go to original source...
    14. Ikawa, I.J., Nishizawa, K., Mira, N.K.: Specifications of several acid phosphatases from plant source.-Nature 230: 939, 1964. Go to original source...
    15. King, M.W., Roberts, E.H.: The Storage of Recalcitrant Seeds-Achievements and Possible Approaches.-International Board for Plant Genetic Resources, Rome 1979.
    16. Koostra, P.T., Harrington, J.F.: Biochemical effects of aging on membrane lipids of Cucumis sativus L. seeds.-Proc. Int. Seed Test Assoc. 34: 329-340, 1969.
    17. Lowry, P.H., Rosebrough, N.J., Farr, A.L., Randall, R.J.: Protein measurement with the Folin phenol reagent.-J. biol. Chem. 193: 265-275, 1951. Go to original source...
    18. Malik, C.P., Singh, M.B. (ed.): Plant Enzymology and Histoenzymology.-Kalyani Publishers, New Delhi 1980.
    19. McCredy, R.M., Guggolz, J., Silviera, V., Owens, H.S.: Determination of starch and amylase in vegetables.-Anal Chem. 22: 1156, 1950. Go to original source...
    20. Mocquot, B., Pradet, A., Litvak, S.: Effect of anoxia on energy charge and protein synthesis in rice embryo.-Plant Physiol. 68: 636-640, 1977. Go to original source...
    21. Nautiyal, A.R., Thapliyal, A.P., Purohit, A.N.: Seed viability in Sal. IV. Protein changes accompanying loss of viability in Shorea robusta.-Seed Sci. Technol. 13: 83-86, 1985.
    22. NKang, A.N.: Some aspects of the biochemical basis of viability of loss in Guilfoylia monostylis seeds.-Seed Sci. Technol. 16: 247-260, 1988.
    23. Pearce, R.S., Abedel Samad, I.M.: Changes in fatty acid content of polar lipids during aging of seeds of peanut Arachis hypogea L.-J. exp. Bot. 31: 1283-1290, 1980. Go to original source...
    24. Powell, A.A., Mattews, S.: Association of phospholipids changes with early stages of seed ageing.-Ann. Bot. 47: 709-712, 1981.
    25. Ram, C., Wiesner, L.R.: Effect of artificial ageing on physiological and biochemical parameters of seed quality in wheat-Seed. Sci. Technol. 16: 579-587, 1988.
    26. Roberts, E.H.: Cytological, genetical and metabolic changes associated with loss of viability.-In: Roberts, E.H. (ed.): Viability of Seeds. Pp. 14-58. Chapman and Hall, London 1972. Go to original source...
    27. Stewart, R.R.C., Bewley, J.D.: Lipid peroxidation associated with accelerated ageing of soybean axes.-Plant Physiol. 65: 245-248, 1980. Go to original source...
    28. Torriani, A.: Alkaline phosphatases from Escherichia coli.-In: Cantoni, G.L., Davies, H. (ed.): Procedure in Nucleic Acid Research. Pp. 212-213. Harper and Row, New York 1967.
    29. Troll, W., Canon, K.: A modified photometric ninhydrin method for the analysis of aminoacids.-J. biol. Chem. 200: 803-811, 1953. Go to original source...
    30. Woodstock, L.W., Grabe, D.F.: Relationship between seed respiration during imbibition and subsequent seedling growth in Zea mays L.,-Plant Physiol. 42: 1071-1076, 1967. Go to original source...

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