biologia plantarum

Biologia plantarum 39:543-553, 1997 | DOI: 10.1023/A:1001701203195

DNA Polymorphism among Rice Somaclones

H. Banerjee¹, V. Chimote¹, S.K. Raina¹

¹ NRC on Plant Biotechnology, IARI, New Delhi, India

Molecular markers were used to detect the influence of high concentrations of 2,4 dichlorophenoxyacetic acid (2,4-D) in the callusing media on DNA variations in regenerated rice plants. Restriction fragment length polymorphism (RFLP), random amplified polymorphic DNA (RAPD) and polymerase chain reaction (PCR) based RFLP analysis were carried out on 12 somaclones of Oryza sativa L. cv. B-370. In vitro culture induced DNA variations were detected in the regenerated plants but the effect of high auxin concentration in the medium could not be revealed. In a second study, fingerprinting of 15 semi-dwarf, high yielding somaclones of B-370 was carried out using RAPD technique. Amplification using 20 random primers produced a total of 167 DNA bands out of which 97 bands were polymorphic. A total of 32 unique DNA bands were detected across all the somaclones and they could be grouped based on their similarity to B-370. RAPD analysis helped to reveal similarity or differences among the somaclones while fingerprinting using additional RAPD markers was not successful.

Keywords: 2,4-D; Oryza sativa; RFLP; RAPD; PCR based RFLP

Subjects: 2,4-dichlorophenoxyacetic acid, DNA variation; DNA polymorphism, rice; Oryza sativa; polymerase chain reaction, rice somaclones; random amplified polymorphic DNA; restriction fragment length polymorphism, rice somaclones; rice, DNA polymorphism among somaclones

Published: December 1, 1997  Show citation