biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 39:605-615, 1997 | DOI: 10.1023/A:1001765522759

Light-induced Changes in Expression of Pathogenesis-related Anionic Peroxidase in Cucumber Seedlings

V. Repka1, I. Fischerova2
1 Laboratory of Molecular Biology and Virology, Complex Research Institute of Viticulture and Enology, Matúąkova 25, Bratislava, Slovakia
2 Department of Biochemistry and Microbiology, Faculty of Chemical Technology, Slovak Technical University, Radlinského 9, Bratislava, Slovakia

The effect of irradiance on the expression of the Cucumis sativus pathogenesis-related (srPRX) anionic peroxidase was studied in germinating seeds at the period when seedlings start to be photosynthetically active. The diversity in the expression patterns of srPRX was observed in both dark- and light-grown seedlings using activity staining and immunoblotting: beside the three srPRX isoenzymes also other three, serologically unrelated, peroxidase isoforms were accumulated in dark-grown seedlings and one in light-grown seedlings. Furthermore, in light-grown seedlings, it was observed a marked difference in the expression of particular srPRX isoenzymes at higher irradiance (up to 260 W m-2, 400 - 700 nm) in comparison to low irradiance. By tissue printing on nitrocellulose paper it was demonstrated that irradiance during germination induced changes in the temporal and spatial distribution of the srPRX.

Keywords: Cucumis; electrophoresis; immunoblotting; localization; tissue printing
Subjects: cucumber, expression of pathogenesis-related anionic peroxidase; Cucumis sativus; expression of pathogenesis-related anionic peroxidase; immunoblotting; pathogenesis-related anionic peroxidase; peroxidase, anionic, pathogenesis; tissue printing

Published: December 1, 1997  Show citation

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Repka, V., & Fischerova, I. (1997). Light-induced Changes in Expression of Pathogenesis-related Anionic Peroxidase in Cucumber Seedlings. Biologia plantarum, 39(4), 605-615. doi: 10.1023/A:1001765522759
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