biologia plantarum

International journal on Plant Life established by Bohumil Nėmec in 1959

Biologia plantarum 41:39-48, 1998 | DOI: 10.1023/A:1001700213655

Cultivar Identification and Relationships in Pisum Sativum L. Based on RAPD and Isoenzymes

P. Samec, Z. Pošvec, J. Stejskal, V. Našinec, M. Griga

Two approaches were used to detect variability within 23 pea cultivars. Eight random amplified polymorphic DNA (RAPD) primers produced 163 bands (114 polymorphic), whereas five isoenzyme systems produced 25 bands (20 polymorphic). Both methods provided good results in finger-printing and identified all cultivars tested. Genetic similarity analysis (UPGMA) gave different results from RAPD and isoenzyme data. This could reflect different properties of both marker types. These data were also analysed using the bootstrap method, which supported the idea of one common progenitor of cultivated peas.

Keywords: biochemical markers; genetic relationships; molecular markers; pea
Subjects: cultivar identification, RAPD and isoenzymes; pea, cultivar identification; random amplified polymorphic DNA, RAPD markers

Published: January 1, 1998  Show citation

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Samec, P., Pošvec, Z., Stejskal, J., Našinec, V., & Griga, M. (1998). Cultivar Identification and Relationships in Pisum Sativum L. Based on RAPD and Isoenzymes. Biologia plantarum, 41(1), 39-48. doi: 10.1023/A:1001700213655
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