biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 41:403-413, 1998 | DOI: 10.1023/A:1001802528068

Chilling-induced changes in membrane fluidity and antioxidant enzyme activities in Coffea arabica L. roots

C.G.S. Queiroz1, A. Alonso2, M. Mares-Guia1, A.C. Magalhães3
1 Departamento de Botânica and Departamento de Bioquimica e Imunologia, UFMG, ICB, Belo Horizonte/MG, Brazil
2 Instituto de Física, UFGO, Goiânia/GO, Brazil
3 Departamento de Fisiologia Vegetal, UNICAMP, IB, Campinas/SP, Brazil

Exposure of coffee to low temperatures caused growth inhibition, changes in metabolic rates, and membrane alterations. Root tissue exposed to 10 °C evolved significantly lower rates of metabolic heat compared with controls grown at 25 °C, and the values were closely associated with the observed root growth inhibition. Electron paramagnetic resonance spectra of intact tissues showed that the spin probe 5-doxylstearic acid was capable to intercalate within the cellular membrane lipids. Indeed, at the depth of the 5th carbon atoms of the alkyl chains, the nitroxide radical detected more rigid membranes in seedlings exposed to 10 °C compared with 25 °C treated samples. Ascorbate peroxidase and catalase activities did not show appreciable changes under chilling conditions, while guaiacol peroxidase activity increased 55 % compared to the control. On the other hand, glutathione reductase activity decreased, in parallel to a significant decline in the capacity to reduce triphenyl-tetrazolium. Our results showed a marked correlation between lipid peroxidation and root tissue damage, which seemed to be associated with increased membrane rigidity.

Keywords: coffee; lipid peroxidation; PPR; stress
Subjects: antioxidant enzyme activities, chilling stress; chilling stress, membrane fluidity; Coffea arabica; coffee, chilling stress; lipid peroxidation, chilling stress

Published: April 1, 1998  Show citation

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Queiroz, C.G.S., Alonso, A., Mares-Guia, M., & Magalhães, A.C. (1998). Chilling-induced changes in membrane fluidity and antioxidant enzyme activities in Coffea arabica L. roots. Biologia plantarum, 41(3), 403-413. doi: 10.1023/A:1001802528068
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