biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 41:547-554, 1998 | DOI: 10.1023/A:1001892300233

Quantitative changes in maize cytoplasmic proteins induced by aluminium

J. Huttová1, L. Tamás1, I. Mistrík1
1 Slovak Academy of Sciences, Dúbravská cesta 14, Institute of Botany, Bratislava, Slovakia

Three-day-old maize seedlings were subjected to 100 µM AlCl3 for 24 h. Cytoplasmic proteins were isolated from root tips, root base and from coleoptiles. After fractionation of cytoplasmic proteins on anion chromatography column Bio-Scale Q2 sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE) analysis was used to monitor Al-induced changes in polypeptide composition of particular fractions. Four (root) and 7 (coleoptile) fractions were eluted from the column with linear 0 - 1.0 M NaCl gradient. In fraction 1 of cytoplasmic proteins from root tips Al induced accumulation of polypeptide with molecular mass of 16 kD and simultaneous reduction of two polypeptides (67.5 and 60 kD). In fraction 1 isolated from mature zone of maize roots Al-induced accumulation of 22 kD polypeptide and reduction of 67.5, 60, and 14 kD polypeptides. Most pronounced changes were revealed in coleoptile. In three protein fractions increased accumulation of polypeptides with molecular mass of 14, 17.5, 20, 24.5, 28, 30, and 37.5 kD were observed. In the remaining three root or four coleoptile fractions of cytoplasmic proteins, no differences were found between Al-treated and control maize seedlings.

Keywords: 3l-induced stress; coleoptile; polypeptide patterns; root; Zea mays L.
Subjects: aluminium, cytoplasmic proteins; coleoptile, maize cytoplasmic proteins; maize, cytoplasmic proteins, aluminium; polypeptide patterns, aluminium stress; root, aluminium stress; Zea mays

Published: May 1, 1998  Show citation

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Huttová, J., Tamás, L., & Mistrík, I. (1998). Quantitative changes in maize cytoplasmic proteins induced by aluminium. Biologia plantarum, 41(4), 547-554. doi: 10.1023/A:1001892300233
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