biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 41:555-564, 1998 | DOI: 10.1023/A:1001844417071

Genotypic differences and alterations of protein patterns of tomato explants under copper stress

H.M. El-Aref1, A.M. Hamada2,*
1 Department of Genetics, Faculty of Agriculture, Assiut University, Assiut, Egypt
2 Department of Botany, Faculty of Science, Assiut University, Assiut, Egypt

In vitro response of six tomato genotypes to different copper concentrations was studied. Cu was toxic to tomato explants at a relatively high concentration (100 µM), which reduced callus growth and shoot regeneration. Peto-86 followed by UC-97-3 were more tolerant to copper than the other genotypes. Cu (100 µM) induced the synthesis of eight new proteins (70.86 - 14.78 kD) in Peto-86 and six in Western Improve (46.43 - 14.78 kD) and UC-97-3 (77.69 - 14.78 kD). Cu-stress reduced the expression of some enzymatic bands of alcohol dehydrogenase and esterase, meanwhile, one peroxidase band at the locus Prx-1 was newly expressed under Cu-treatment.

Keywords: alcohol dehydrogenase; esterase; Lycopersicon esculentum; peroxidase; tissue culture
Subjects: alcohol dehydrogenase, copper stress; callus (tissue) culture, copper stress; copper stress, protein patterns; esterase, copper stress; Lycopersicon esculentum; peroxidase, copper stress; protein patterns of tomato explants, genotypes, copper

Published: May 1, 1998  Show citation

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El-Aref, H.M., & Hamada, A.M. (1998). Genotypic differences and alterations of protein patterns of tomato explants under copper stress. Biologia plantarum, 41(4), 555-564. doi: 10.1023/A:1001844417071
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