biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 42:149-154, 1999 | DOI: 10.1023/A:1002141509131

Evolution of Ethylene and Methane in Relation to Somatic Embryogenesis in Chickpea

S.K. Guru1, R. Chandra1, A. Raj1, S. Khetrapal1, R. Polisetty1
1 Plant Tissue Culture Laboratory, Division of Plant Physiology, I.A.R.I., New Delhi, India

In four genotypes of chickpea (Cicer arietinum L.) BG 362, BG 372, BG 329 and C235 the relationship between somatic embryogenesis of leaf explants and ethylene and methane evolution was studied. In BG 362, which was more embryogenic than other genotypes, a higher ethylene:methane ratio of 5.8:1 at day one after inoculation in the induction medium and a lower ethylene:methane ratio of 2.89:1 in the maturation medium was found. On the contrary, in BG 372 with the least embryogenic potential, a lower ethylene:methane ratio of 1.7:1 in the induction medium and a higher ethylene:methane ratio of 4:1 in the maturation medium was found. Thus, these ratios in induction and maturation stages seems to be markers for embryogenesis in leaf explants of chickpea.

Keywords: Cicer arietinum; induction stage; maturation stage
Subjects: chickpea genotypes, somatic embryogenesis; Cicer arietinum; ethylene/methane in induction/maturation stages as markers for embryogenesis; somatic embryogenesis, ethylene/methane as markers

Published: July 1, 1999  Show citation

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Guru, S.K., Chandra, R., Raj, A., Khetrapal, S., & Polisetty, R. (1999). Evolution of Ethylene and Methane in Relation to Somatic Embryogenesis in Chickpea. Biologia plantarum, 42(1), 149-154. doi: 10.1023/A:1002141509131
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    References

    1. Babbar, S.B., Gupta, C.: Putative role of ethylene in Datura metel microspore embryogenesis.-Physiol. Plant. 68: 141-144, 1986. Go to original source...
    2. Chandra, R., Chatrath, A., Polisetty, R., Khetrapal, S.: Differentiation of in vitro grown explants of chickpea (Cicer arietinum L.).-Indian J. Plant Physiol. 36: 121-124, 1993.
    3. Chandra, R., Khetrapal, S., Polisetty, R.: Effect of plant growth regulators on evolution of ethylene and methane by different explants of chickpea.-Biol. Plant. 40: 337-343, 1997/98. Go to original source...
    4. Chi, G.L., Pua, F.C., Goh, C.I.: Role of ethylene on de novo shoot regeneration from cotyledonary explants of Brassica campestris ssp. Pekinensis (Lour) Olsson in vitro.-Plant Physiol. 96: 178-183, 1991. Go to original source...
    5. Cho, U.H., Kasha, K.J.: Ethylene production and embryogenesis from anther cultures of barley (Hordeum vulgare).-Plant Cell Rep. 8: 415-417, 1989. Go to original source...
    6. Evans, J.M., Batty, N.P.: Ethylene precursors and antagonists increase embryogenesis of Hordeum vulgare L. anther culture.-Plant Cell Rep. 13: 676-678, 1994. Go to original source...
    7. Garcia, F.G., Einset, J.W.: Ethylene and ethane production in 2.4-D treated and salt treated tobacco tissue cultures.-Ann. Bot. 51: 287-295, 1983. Go to original source...
    8. Hagege, D., Keevers, C., Geuns, J., Gaspar, T.: Ethylene production and polyamine content of fully habituated sugarbeet calli.-J. Plant Physiol. 143: 722-725, 1994. Go to original source...
    9. Huxter, T.J., Thorpe, T.A., Reid, D.M.: Shoot initiation in light and dark grown tobacco callus: the role of ethylene.-Physiol. Plant. 53: 319-326, 1981. Go to original source...
    10. Khehra, G.S., Mathias, R.J.: The interaction of genotype, explant and media on the regeneration of shoots from complex explants of Brassica napus L.-J. exp. Bot. 43: 1413-1418, 1992. Go to original source...
    11. Kumar, P.P., Reid, D.M., Thorpe, T.A.: The role of ethylene and carbon dioxide in differentiation of shoot buds in excised cotyledons of Pinus radiata in vitro.-Physiol. Plant. 69: 244-252, 1987. Go to original source...
    12. Lazzeri, P.A., Hilderbrand, D.F., Sunega, J., Williams, E.G., Collins, G.B.: Soybean somatic embryogenesis: interaction between sucrose and auxin.-Plant Cell Rep. 7: 517-520, 1988. Go to original source...
    13. Miller, R.A., Pengelly, W.L.: Ethylene production by shoot forming and unorganised crown gall tumour tissues of Nicotiana and Lycopersicon cultured in vitro.-Planta 161: 418-428, 1984. Go to original source...
    14. Mustafa, M.D., Parthasarathy, M., Mallaian, B.: Methyl methane sulphonate (MMS) induced multiple shoot from cotyledon cultures of cucumber (Cucumis sativa L. var. Poinsettl).-Adv. Plant Sci. 4: 419-422, 1991.
    15. Palmer, C.E.: Enhanced shoot regeneration from Brassica campesiris by silver nitrate.-Plant Cell Rep. 11: 541-545, 1992. Go to original source...
    16. Patil, P., Chandra, R., Khetrapal, S., Polisetty, R.: Influence of polyamines and ethylene inhibitors on somatic embryo induction in chickpea (Cicer arietinum L.).-J. Plant Physiol. 3: 26-34, 1998.
    17. Pua, E.C., Sim, G.E., Chi, C.L., Kong, L.F.: Synergistic effect of ethylene inhibitors and putrescine on shoot regeneration from hypocotyl explants of Chinese radish Raphanus sativus L. var. longipinnatus Bailey in vitro.-Plant Cell Rep. 15: 685-690, 1996. Go to original source...
    18. Purnhauser, L., Medgyesy, P., Czako, M., Dix, P.J., Marton, L.: Stimulation of shoot regeneration in Triticum aestivum and Nicotiana plumbaginifolia tissue cultures using the ethylene inhibitor AgNO3.-Plant Cell Rep. 6: 1-4, 1987. Go to original source...
    19. Robinson, K.E.P., Adams, D.O.: The role of ethylene in the regeneration of Helianthus annuus (sunflower) plants from callus.-Physiol. Plant. 71: 151-156, 1987. Go to original source...
    20. Roustan, J.P., Latche, A., Fallot, J.: Control of carrot somatic embryogenesis by AgNO3, an inhibitor of ethylene action: effect on arginine decarboxylase activity.-Plant Sci. 67: 89-95, 1990. Go to original source...
    21. Sudarsana Rao, G.V.: Factors affecting shoot regeneration in pigeonpea (Cajanus cajan L.).-Ph.D. Thesis. Post Graduate School, Indian Agricultural Research Institute, New Delhi 1997.
    22. Tisserat, B., Murashige, T.: Effect of ethephon, ethylene and 2,4-D on asexual embryogenesis in vitro.-Plant Physiol. 60: 437-439, 1977. Go to original source...
    23. Williams, J., Pink, D.A.C., Biddington, N.L.: The effect of silver nitrate on growth and plant regeneration from Brassica oleracea var. gemmifera callus.-Plant Cell Tissue Organ Cult. 21: 61-66, 1990. Go to original source...

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