biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 42:297-302, 1999 | DOI: 10.1023/A:1002177122335

Protein Profiles of Somatic Embryos and Regenerated Plants from NaCl Selected and Control Cultures of Orchardgrass

S. Dutta Gupta1
1 Department of Agricultural and Food Engineering, Indian Institute of Technology, Kharagpur, India

The protein profile of cells of control somatic embryos was compared to that of embryos that have become selected and maintained on 200 mM NaCl in order to detect salt inducible proteins. Two proteins (60 and 51.5 kDa) were more abundant in the selected embryos and one protein with molecular mass 18 kDa was unique to the selected embryos. Enhanced content of 27 kDa protein was observed in all somatic embryos indicating its involvement in the embryonal state. Similar pattern of salt inducible proteins in selected somatic embryos and the plantlets regenerated from such embryos was found.

Keywords: Dactylis glomerata; NaCl tolerance; plant regeneration; somatic embryogenesis
Subjects: amylose/amylopectin, microanalytical determination; Dactylis glomerata; determination, amylose/amylopectin, starch; in vitro culture, regeneration; in vitro culture, somatic embryogenesis; NaCl tolerance, somatic embryogenesis; orchardgrass, somatic embryogenesis; pea, seed, starch; Pisum sativum; protein profiles, somatic embryos, regenerants; salinity, somatic embryogenesis; somatic embryogenesis, NaCl stress; starch, microanalytical determination; tolerance, NaCl, somatic embryogenesis

Published: September 1, 1999  Show citation

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Dutta Gupta, S. (1999). Protein Profiles of Somatic Embryos and Regenerated Plants from NaCl Selected and Control Cultures of Orchardgrass. Biologia plantarum, 42(2), 297-302. doi: 10.1023/A:1002177122335
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