biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 42:341-347, 1999 | DOI: 10.1023/A:1002452900035

Isoenzyme Expression During Root and Shoot Formation in Solanum Nigrum

A.M. Hassanein1, A.M. Ahmed2, A.I.I. Abed-El-Hafez1, D.M. Soltan1
1 Botany Department, Faculty of Science, South Valley University, Sohag, Egypt
2 Botany Department, Faculty of Science, Assuit University, Assuit, Egypt

In Solanum nigrum, the initiation of root primordia occurred directly on stem explants or microshoots cultured on half strength MS medium supplemented with 1 mg dm-3 indolebutyric acid (IBA), indole-3-acetic acid, or naphthalene acetic acid, IBA being most effective. Initiation of shoot primordia occurred on B5 medium supplemented with 0.5 mg dm-3 benzylaminopurine (BAP) or kinetin; BAP was more active. During shoot formation, the increased content and the expression of new isoenzymes of peroxidase (POX), esterase and malate dehydrogenase was found. On the other side, expression of new indophenol peroxidase isoenzyme was detected during root formation. Organogenesis of S. nigrum has no effect on glutamate oxaloacetate transaminase and glutamate dehydrogenase content. Differential expression of POX could be detected between basal and upper side of explants.

Keywords: auxins; cytokinins; esterase; peroxidase; glutamate oxaloacetate transaminase; glutamate dehydrogenase; malate dehydrogenase; indophenol peroxidase
Subjects: esterase, root and shoot formation; glutamate dehydrogenase, root and shoot formation; glutamate oxaloacetate transaminase, root/shoot formation; growth regulators, root/shoot formation; indophenol peroxidase, root and shoot formation; isoenzyme expression, root and shoot formation; malate dehydrogenase, root/shoot formation; peroxidase, root and shoot formation; Solanum nigrum

Published: November 1, 1999  Show citation

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Hassanein, A.M., Ahmed, A.M., Abed-El-Hafez, A.I.I., & Soltan, D.M. (1999). Isoenzyme Expression During Root and Shoot Formation in Solanum Nigrum. Biologia plantarum, 42(3), 341-347. doi: 10.1023/A:1002452900035
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