biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 42:515-523, 1999 | DOI: 10.1023/A:1002698511484

Morphogenesis in Helianthus Tuberosus: Genotypic Influence and Increased Totipotency in Previously Regenerated Plants

R. Bianchi1, M. Fambrini1, C. Pugliesi1
1 Dipartimento di Biologia delle Piante Agrarie, Sezione di Genetica, Pisa, Italy

Leaf tissues of 38 genotypes, derived from four accessions, of the hexaploid species Helianthus tuberosus (2n=6x=102) responded to growth regulators (BA, NAA) chiefly by forming callus, while aventitious organogenesis or somatic embryogenesis were induced occasionally. A remarkable regeneration frequency (about 30 %) was achieved only from leaves of genotype HTPI-15. Explants of many regenerated plants of HTPI-15 subjected to a second culture cycle in vitro displayed a high morphogenetic potential (regeneration frequency > 90 %). White globular structures were initiated on the adaxial surface of these leaves without a callus phase. Somatic embryogenesis was asynchronous and embryoids, of different developmental stage, were simultaneously detected on each explant. Although many embryos developed single or malformed cotyledons or germinated precocciously, without the differentiation of a complete root system, phenotypically normal plants were regenerated after rooting on regulator-free half-strength MS medium.

Keywords: cellular determination; competence; somatic embryogenesis
Subjects: accessions of different geographical origin, Helianthus; Helianthus tuberosus × H. annuus genotypes; morphogenetic competence, in Helianthus tuberosus and H. annuus genotypes; regeneration frequency, Helianthus tuberosus and H.annuus genotypes; somatic embryogenesis, Helianthus tuberosus × H. annuus genotypes; totipotency in previously regenerated plants

Published: December 1, 1999  Show citation

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Bianchi, R., Fambrini, M., & Pugliesi, C. (1999). Morphogenesis in Helianthus Tuberosus: Genotypic Influence and Increased Totipotency in Previously Regenerated Plants. Biologia plantarum, 42(4), 515-523. doi: 10.1023/A:1002698511484
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