biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 43:13-18, 2000 | DOI: 10.1023/A:1026534425741

Molecular Characterization of a Hybrid Zone between Orchis mascula and O. pauciflora in Southern Italy

G. Pellegrino1, P. Caputo2, S. Cozzolino3, B. Menale3, A. Musacchio1
1 Dipartimento di Ecologia, Università della Calabria, Cosenza, Italy
2 Dipartimento di Biologia Vegetale, Università di Napoli Federico II, Napoli, Italy
3 Orto Botanico, Università di Napoli Federico II, Napoli, Italy

A small population (16 individuals) of Orchis × colemanii Cortesi, a natural hybrid between O. mascula (L.) L. and O. pauciflora Ten. was sampled from a locality near Sassano (National Park of Cilento, province of Salerno, Southern Italy) in order to assess its genetic structure. Filter hybridizations of O. × colemanii, O. mascula and O. pauciflora DNAs against a PCR-amplified ribosomal fragment indicated that all the hybrid individuals in study have the ribosomal repeats of both parents, even if not always combined in equal proportions, and that no specimen morphologically identified as O. mascula or O. pauciflora possessed detectable rDNA of the other species. The majority of the hybrid specimens (12 individuals) showed a preponderance of O. mascula rDNA, three had approximately equal proportions of parental repeats and one had a preponderance of O. pauciflora rDNA. As the parental species have different amounts of rDNA, as inferred from dot blot hybridization, it has been deduced that the 12 specimens may represent F1 or successive, presumably non-recombinant progenies, while the last four specimens may represent either F2 genotypes or backcrosses with O. pauciflora.

Keywords: dot blot; hybridization area; introgression; Orchis × colemanii
Subjects: dot blot hybridization; hybrids, Orchis, molecular traits; introgression, Orchis hybrids; Orchis × colemanii; Orchis mascula × O. pauciflora hybrids, molecular traits

Published: March 1, 2000  Show citation

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Pellegrino, G., Caputo, P., Cozzolino, S., Menale, B., & Musacchio, A. (2000). Molecular Characterization of a Hybrid Zone between Orchis mascula and O. pauciflora in Southern Italy. Biologia plantarum, 43(1), 13-18. doi: 10.1023/A:1026534425741
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