biologia plantarum

Biologia plantarum 43:19-23, 2000 | DOI: 10.1023/A:1026586409811

Are the T-DNA Mutants Amenable to Standard Recombination Analysis?

J. Relichová¹, J. Řepková¹

¹ Department of Genetics and Molecular Biology, Faculty of Science, Masaryk University, Brno, Czech Republic e-mail

Genetic analysis with T-DNA mutants often brings difficulties resulting from instability of the transgenic phenotype. In this work three different Arabidopsis thaliana T-DNA embryonic lethals and one T-DNA morphological mutant were analyzed in F2 progeny after 15 different crosses with marker lines for individual chromosomes. F2 analysis of 44 segregation ratios revealed segregation distortion of similar character consisting in abnormal excess of nontransgenic plants to the detriment of transgenic ones. We quantified this phenotypic drift (d) on the basis of phenotypic ratios given the respective formulas. The d values indicate the rate of F1 gametes which loose the T-DNA mutation or ability of its expression. The obtained d value were relatively high, 0.4 to 0.9 for individual crosses. It makes the standard recombination analysis with insertional mutants very problematic or even impossible.

Keywords: Arabidopsis; F2 recombination; phenotypic drift

Subjects: Arabidopsis thaliana; F2 recombination, Arabidopsis; mutants, T-DNA, Arabidopsis; phenotypic drift, Arabidopsis; recombination analysis, Arabidopsis

Published: March 1, 2000  Show citation