biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 43:105-112, 2000 | DOI: 10.1023/A:1026515316171

Serologically-Related Anionic Peroxidases from Petunia and Cucumber can Substitute Flavonoid Antioxidants

V. Repka1, I. Fischerová1
1 Laboratory of Molecular Biology and Virology, C.R.I.V.E., Bratislava, Slovakia e-mail

In this study we have investigated whether naturally occurring flavonoid-deficient mutant Red Star of Petunia hybrida is capable of metabolizing H2O2 by invoking other antioxidant enzyme system. We demonstrated that reduced flower pigmentation due to a reduction in the chalcone synthase mRNA expression results in strong H2O2 accumulation accompanied by the induction of a specific set of anionic peroxidase (PRX), serologically-related to main cucumber srPRX. We found correlation between rate of H2O2 accumulation and qualitative, as well as quantitative changes in the srPRX expression which seems to be determined by flower phenotype. In detached flower buds cultured in vitro both abscisic acid and anther extirpation prevented anthocyanin pigmentation, and thus flavonoid biosynthesis, resulting in a marked accumulation of immunoprecipitable srPRX. In contrast, pigmented flowers cultivated under the same conditions did not accumulate corresponding srPRX. The results suggest that a specific set of anionic PRX can substitute for the absence of flavonoid antioxidants.

Keywords: abscisic acid; chalcone synthase; Cucumis; electrophoresis; immunoblotting; in vitro culture; magnetic immunoprecipitation
Subjects: abscisic acid, flavonoid biosynthesis; chalcone synthase, flower pigmentation; cucumber, serologically-related anionic peroxidases; Cucumis sativus; flavonoid antioxidants, peroxidases, ABA; immunoblotting, flower proteins; in vitro culture, flowering; magnetic immunoprecipitation, flower proteins; peroxidase, flavonoid antioxidants; Petunia hybrida; serologically-related anionic peroxidases

Published: March 1, 2000  Show citation

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Repka, V., & Fischerová, I. (2000). Serologically-Related Anionic Peroxidases from Petunia and Cucumber can Substitute Flavonoid Antioxidants. Biologia plantarum, 43(1), 105-112. doi: 10.1023/A:1026515316171
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