biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 43:121-124, 2000 | DOI: 10.1023/A:1026519417080

Effect of Auxins on in vitro Rooting of Plumbago Zeylanica: Peroxidase Activity as a Marker for Root Induction

C. Saxena1, S. Samantaray2, G.R. Rout1, P. Das1
1 Plant Tissue Culture Laboratory, Regional Plant Resource Centre, Bhubaneswar-, India
2 Plant Physiology and Biochemistry Laboratory, Regional Plant Resource Centre, Bhubaneswar-, India

Induction of rooting in the microshoots of Plumbago zeylanica was achieved on halfstrength basal Murashige and Skoog's medium supplemented with 0.25 mg dm-3 indole-3-butyric acid. Rooting was totally inhibited when the microshoots were cultured in vitro under continuous light, however, maximum percentage of microshoots rooted when incubated in continuous light for 4 weeks before transfer to the rooting media. Peroxidase activity increased markedly during root induction indicating a key role of peroxidase in rooting of microshoots of Plumbago zeylanica in vitro.

Keywords: medicinal plant; root initiation; tissue culture
Subjects: in vitro culture, rooting, auxins; in vitro culture, tissue culture, microshoots; medicinal plants, in vitro culture; peroxidase, root induction marker; Plumbago zeylanica; root initiation, peroxidase

Published: March 1, 2000  Show citation

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Saxena, C., Samantaray, S., Rout, G.R., & Das, P. (2000). Effect of Auxins on in vitro Rooting of Plumbago Zeylanica: Peroxidase Activity as a Marker for Root Induction. Biologia plantarum, 43(1), 121-124. doi: 10.1023/A:1026519417080
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    References

    1. Baraldi, R., Rossi, F., Lercari, B.: In vitro shoot development of Prunus GF 6652: interaction between light and benzyladenine.-Plant Physiol. 74: 440-443, 1988. Go to original source...
    2. Berthon, J.Y., Maldiney, R., Sotta, B., Gaspar, T., Boyer, N.: Endogenous levels of plant hormones during the course of adventitious rooting in cuttings of Sequoiadendron giganteum in vitro.-Biochem. Physiol. Pflanz. 184: 405-412, 1989. Go to original source...
    3. Berthon, J.Y., BenTahar, S., Gaspar, T., Boyer, N.: Rooting phases of shoots of Sequoiadendron giganteum in vitro and their requirements.-Plant Physiol. Biochem. 28: 631-638, 1990.
    4. Bergmeyer, H.U., Gaweh, K., Grassl, M.: Enzymes as Biochemical Reagents.-In: Bergmeyer, H.U. (ed.): Methods in Enzyme Analysis. Pp. 425-522. Academic Press, New York 1974.
    5. Blakesley, D., Weston, G.D., Hau, J.F.: The role of endogenous auxin in root initiation. Part-I: Evidence from studies on auxin application and analysis of endogenous levels.-Plant Growth Regul. 10: 341-353, 1991. Go to original source...
    6. Bradford, M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.-Anal. Biochem. 72: 248-254, 1976. Go to original source...
    7. Gaspar, T., Kevers, C., Hausman, J.F., Berthon, J.Y., Ripetti, V.: Practical uses of peroxidase activity as a predictive marker of rooting performance of micropropagated shoots.-Agronomie 12: 757-765, 1992. Go to original source...
    8. Gaspar, T., Kevers, C., Hausman, J.F., Berthon, J.Y., Ripetti, V.: Peroxidase activity and endogenous free auxin during adventitious root formation.-In: Lumsden, P.J., Nicholas, J.R., Davies, W.J. (ed.): Physiology, Growth and Development of Plants in Culture. Pp. 289-298. Kluwer Academic Publishers, Dordrecht 1994. Go to original source...
    9. Gaspar, T., Penel, C., Greppin, H.: Do rooting induction and flowering evocation involve a similar interplay between indoleacetic acid, putrescine and peroxidases.-In: Greppin, H., Penel, C., Simon, P. (ed.): Travelling Shot on Plant Development. Pp. 35-49. University of Geneva, Geneva 1997.
    10. Hausman, J.F.: Changes in peroxidase activity, auxin level and ethylene production during root formation by poplar shoots raised in vitro.-Plant Growth Regul. 13: 263-268, 1993. Go to original source...
    11. Hausman, J.F., Evers, D., Kevers, C., Gaspar, T.: Internal controls of root induction in poplar shoots raised in vitro.-Angew. Bot. 71: 104-107, 1997.
    12. Jayaraman, K.S.: India seeks scientific basis of traditional remedies.-Nature 326: 323, 1987. Go to original source...
    13. Kevers, C., Hausman, J.F., Faivre-Rampant, O., Evers, D., Gaspar, T.: Hormonal control of adventitious rooting: progress and questions.-Angew. Bot. 71: 71-79, 1997.
    14. Krishnaswamy, M., Purushothaman, K.K.: Plumbagin, a study of its anticancer, antibacterial and antifungal properties.-Indian J. exp. Biol. 18: 876-877, 1980.
    15. Marascuilo, L.A., McSweeney, M.: Post-Hoc multiple comparisons in sample preparations for test of homogeneity.-In: McSweeney, M., Marascuilo, L.A. (ed.): Non-parametric and Distribution Free Methods the Social Sciences. Pp. 141-147. Books/Cole Publ. 1977.
    16. Moncousin, C., Gaspar, T.: Peroxidase as a marker for rooting improvement of Cynara scolymus L. cultured in vitro.-Biochem. Physiol. Pflanz. 178: 263-271, 1983. Go to original source...
    17. Moncousin, C., Favre, J.M., Gaspar, T.: Changes in peroxidase activity and endogenous IAA levels during adventitious root formation in vine cuttings.-In: Kutáček, M., Bandurski, R.S., Krekule, J. (ed.): Physiology and Biochemistry of Auxins in Plants. Pp. 331-337. Academia, Praha 1988.
    18. Murashige, T.: Plant propagation through tissue culture.-Annu. Rev. Plant Physiol. 25: 135-166, 1974. Go to original source...
    19. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures.-Physiol. Plant. 15: 473-497, 1962. Go to original source...
    20. Quoirin, M., Boxus, P., Gaspar, T.: Root initiation and isoperoxidase of stem tip cuttings from mature Prunus plants.-Physiol. vég. 12: 165-174, 1974.
    21. Rout, G.R., Das, P.: Somatic embryogenesis in Simarouba glauca.-Plant Cell Tissue Organ Cult. 37: 79-81, 1994. Go to original source...
    22. Rival, A., Bernard, F., Mathieu, Y.: Changes in peroxidase activity during in vitro rooting of oil palm (Elaeis guineensis Jacq.).-Sci. Hort. 71: 103-112, 1997. Go to original source...
    23. Samantaray, S., Rout, G.R., Das, P.: An in vitro studies of organogenesis in Trema orientalis (Blume) Linn.-Plant Sci. 105: 87-94, 1995. Go to original source...
    24. Saxena, C., Rout, G.R., Das, P.: Micropropagation of Psoralea corylifolia.-J. med. aromat. Plant Sci. 20: 15-18, 1998.
    25. Seibert, M., Kadkade, P.G.: Environmental factors: a light.-In: Staba, E.J. (ed.): Plant Tissue Culture as a Source of Biochemicals. Pp. 123-141. CRC Press, Boca Raton 1980.

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