biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 43:125-128, 2000 | DOI: 10.1023/A:1026571401150

Effect of Different Saccharides on Viability of Isolated Microspores and Androgenic Induction in Zea Mays

B. Obert1, A. Pre»ová1, B. Büter2, J.E. Schmid2
1 Institute of Plant Genetic and Biotechnology, Slovak Academy of Sciences, Nitra, Slovak Republic
2 Institute of Plant Science, Swiss Federal Institute of Technology (ETH) Zürich, Lindau, Switzerland

Sucrose, glucose, fructose, and melibiose in different concentrations and combinations in the induction media influenced the viability of the isolated maize microspores and the formation of multinuclear structures. The induction of multinuclear structures on media containing combination of sucrose, fructose and glucose was lower than on media only with sucrose. In media containing melibiose alone or in combination with sucrose, no induction of multinuclear structures was found, however, microspore viability was improved.

Keywords: gametic embryogenesis; haploid; maize
Subjects: gametic embryogenesis, in vitro culture, microspores; haploid, in vitro culture, microspores; in vitro culture, gametic embryogenesis, microspores; in vitro culture, microspores, saccharides; maize, in vitro culture, microspores; microspore viability, in vitro culture; multinuclear structures, induction, saccharides; Zea mays

Published: March 1, 2000  Show citation

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Obert, B., Pre»ová, A., Büter, B., & Schmid, J.E. (2000). Effect of Different Saccharides on Viability of Isolated Microspores and Androgenic Induction in Zea Mays. Biologia plantarum, 43(1), 125-128. doi: 10.1023/A:1026571401150
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