biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 43:137-139, 2000 | DOI: 10.1023/A:1026527718897

Effect of Paclobutrazol on Soluble Sugars and Starch Content of de novo Regenerating Potato Stem Explants

M. Grospietsch1, H. Lipavská2, J. Opatrná3
1 Research Institute of Crop Production, Prague 6, Czech Republic e-mail
2 Department of Plant Physiology, Charles University, Prague 2, Czech Republic
3 Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Prague 6, Czech Republic

Changes in the content of soluble sugars and starch were determined during the first phase of de novo organogenesis in stem internode segments (SIS), taken either from control plants or from the plants grown on medium with 10-8 M paclobutrazol (PBZ). Transient accumulation of soluble sugars was observed in both variants during the first two days. Control SIS accumulated higher amount [22.0 mg g-1(f.m.)] of soluble sugars than the PBZ pretreated SIS [15.2 mg g-1(f.m.)]. PBZ variant showed four times higher starch content at the start of experiment in comparison with the control. Both variants accumulated starch during cultivation until the beginning of regeneration.

Keywords: growth retardants; organogenesis; stem intermode segments
Subjects: growth retardants, de novo organogenesis; in vitro culture, de novo organogenesis; organogenesis de novo, stem segments, saccharides; paclobutrazol, saccharides, stem explants; potato, stem internode segments, de novo organogenesis; Solanum tuberosum; stem internode segments, de novo organogenesis

Published: March 1, 2000  Show citation

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Grospietsch, M., Lipavská, H., & Opatrná, J. (2000). Effect of Paclobutrazol on Soluble Sugars and Starch Content of de novo Regenerating Potato Stem Explants. Biologia plantarum, 43(1), 137-139. doi: 10.1023/A:1026527718897
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