Biologia plantarum 43:145-148, 2000 | DOI: 10.1023/A:1026531819806
A Low Transmissibility of Hop Latent Viroid through a Generative Phase of Humulus lupulus L.
- 1 Institute of Plant Molecular Biology, Academy of Sciences of the Czech Republic, České Budějovice, Czech Republic e-mail
- 2 Hop Research Company, Žatec, Czech Republic
A significant reduction of hop latent viroid (HLVd) content during the generative phase of hop was detected using reverse transcriptase-polymerase chain reaction (RT PCR) and molecular hybridisation methods. A low transmissibility of HLVd through seed may be a feature valuable for the selection and maintenance of viroid-free hybrid hops.
Keywords: viroid elimination; seed transmissibility; hybrid hops; RT PCR; dsRNAse; molecular hybridisation
Subjects: hop latent viroid; Humulus lupulus; hybrid hops, hop latent viroid; molecular hybridisation, hop latent viroid; polymerase chain reaction, PCR; polymerase chain reaction, PCR, reverse transcriptase-polymerase chain reaction, RT PCR; reverse transcriptase-polymerase chain reaction, RT PCR; RNAse, dsRNAse; seed transmissibility; transcriptase-polymerase chain reaction, reverse, RT PCR; viroid elimination, hybrid hops
Published: March 1, 2000 Show citation
References
- Adams, A.N., Barbara, D.J., Morton, A.: Effects of hop latent viroid on weight and quality of the cones of the hop cultivar Wye Challenger.-Ann. appl. Biol. 118(Suppl.): 126-127, 1991.
- Barbara, D.J., Morton, A., Adams, A.N., Green, C.P.: Some effects of hop latent viroid on two cultivars of hop (Humulus lupulus) in the UK.-Ann. appl. Biol. 117: 359-366, 1990.
Go to original source... - Bennett, C.W.: Seed transmission of plant viruses.-Adv. Virus Res. 14: 221-261, 1969.
Go to original source... - Diener, T.O.: The Viroids.-Plenum Press, New York-London 1987.
Go to original source... - Kryzyński, S., Paduch-Cichal, E., Skrzeckowski, L.J.: Transmission of three viroids through seed and pollen of tomato plants.-J. Phytopathol. 121: 51-57, 1988.
Go to original source... - Matoušek, J., Trněná, L., Svoboda, P., Oriniaková, P., Lichtenstein, C.P.: The gradual reduction of viroid levels in hop mericlones following heat therapy: A possible role for a nuclease degrading dsRNA.-Biol. Chem. Hoppe-Seyler 376: 715-721, 1995.
Go to original source... - Polívka, H., Staub, U., Gross, H.J.: Variation of viroid profiles in individual grapevine plants: novel grapevine yellow speckle viroid 1 mutants show alterations of hairpin I.-J. gen. Virol. 77: 155-161, 1996.
Go to original source... - Puchta, H., Ramm, K., Sänger, H.L.: The molecular structure of hop latent viroid (HLV), a new viroid occurring world-wide in hops.-Nucl. Acids Res. 16: 4197-4216, 1988.
Go to original source... - Singh, R.P.: Seed transmission of potato spindle tuber virus in tomato and potato.-Amer. Potato J. 47: 225-227, 1970.
Go to original source... - Singh, R.P. Boucher, A., Somerwille, T.H.: Evaluation of chemicals for disinfection of laboratory equipment exposed to potato spindle tuber viroid.-Amer. Potato J. 66: 239-246, 1989.
Go to original source... - Singh, R.P., Boucher, A., Singh, A.: High incidence of transmission and occurrence of a viroid in commercial seeds of Coleus in Canada.-Plant Dis. 75: 184-187, 1991.
Go to original source... - Singh, R.P. Boucher, A., Somerwille, T.H.: Detection of potato spindle tuber viroid in the pollen and various parts of potato plant pollinated with viroid-infected pollen.-Plant Dis. 76: 951-953, 1992.
Go to original source... - Wallace, J.M., Drake, R.J.: A high rate of seed transmission of avocado sun-blotch virus from symptomless trees and the origin of such trees.-Phytopathology 52: 237-241, 1962.
- Yaguchi, S., Takahashi, T.: Survival of hop stunt viroid in the hop garden.-Phytopathol. Z. 109: 32-44, 1984.
Go to original source...



