biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 43:173-177, 2000 | DOI: 10.1023/A:1002727521624

The Effects of Photoperiod, Glucose and Gibberellic Acid on Growth In Vitro and Flowering of Chenopodium Murale

A. Mitrović1, B. Živanović1, Lj. Ćulafić2
1 Center for Multidisciplinary Studies, University of Belgrade, Belgrade, Yugoslavia
2 Institute of Botany, Faculty of Science, University of Belgrade, Belgrade, Yugoslavia

In vitro culture of long-day plant Chenopodium murale L was established. The effects of photoperiod, glucose and gibberellic acid (GA3) on flowering and growth in vitro were investigated. Oscillatory changes of photoperiodic sensitivity were noticeable with regard to plant age. The plants induced at the phase of the 1st and the 3rd pair of leaves flowered to higher degree than those induced at the phase of 2nd pair. Plants induced at the phase of the 1st pair of leaves flowered to 17 % on 5 % glucose-containing medium and the addition of 5 mg dm-3 GA3 resulted in maximum flowering (43 %). Neither glucose nor GA3 were able to compensate for photoperiodic requirements for flowering. Hypocotyl growth was decreased and the 1st internode elongation and development of leaves were increased due to inductive photoperiodic conditions, as compared to non-inductive ones.

Keywords: hypocotyl elongation; 1st internode elongation; leaf initiation; photoperiodic sensitivity
Subjects: Chenopodium murale; flowering, in vitro culture; gibberellic acid, photoperiod, in vitro culture; hypocotyl elongation; in vitro culture, photoperiodic sensitivity; internode elongation; leaf initiation; photoperiodic sensitivity, in vitro culture

Published: August 1, 2000  Show citation

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Mitrović, A., Živanović, B., & Ćulafić, L. (2000). The Effects of Photoperiod, Glucose and Gibberellic Acid on Growth In Vitro and Flowering of Chenopodium Murale. Biologia plantarum, 43(2), 173-177. doi: 10.1023/A:1002727521624
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