biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 43:211-215, 2000 | DOI: 10.1023/A:1002743908420

Composition of Esterase and Peroxidase Isoenzyme Complex, Zein-2 Protein Fraction, and SDS-Protein Complex of Zea Mays L. × Tripsacum Dactyloides L. Hybrids and Parents

V. Tsanev1, L. Todorova1, S. Rocheva1
1 Acad. D. Kostoff Institute of Genetics, Bulgarian Academy of Sciences, Sofia -, Bulgaria

The patterns of esterase and peroxidase isoenzymes, subunits of zein-2 fraction and protomers of SDS-protein complex of Zea mays L. × Tripsacum dactyloides L. hybrids and their parents were compared. The study has been made to detect specific to Tripsacum isoesterases and isoperoxidases, zein subunits and SDS-protein protomers which could be used as markers for introgression of gene loci encoding these proteins from Tripsacum into hybrids of Tripsacum with Zea mays. Isoesterases and isoperoxidases as well protomers of SDS-protein complex specific to Tripsacum were detected in all hybrids analyzed. Zein subunits, specific to Tripsacum were detected in some of the analyzed hybrids which i that introgression frequency of the loci encoding proteins studied was different. Chromosome counts taken on the examined hybrids showed the addition of 9 - 13 Tripsacum chromosomes to maize chromosome complement.

Keywords: chromosome complement; electrophoresis; introgression frequency; maize
Subjects: chromosome complement, maize; esterase isozyme complex; introgression frequencies, maize; peroxidase isozyme complex; SDS-protein complex; Tripsacum ' Zea hybrids and parents; Zea mays ' Tripsacum hybrids and parents; zein-2 protein fraction

Published: August 1, 2000  Show citation

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Tsanev, V., Todorova, L., & Rocheva, S. (2000). Composition of Esterase and Peroxidase Isoenzyme Complex, Zein-2 Protein Fraction, and SDS-Protein Complex of Zea Mays L. × Tripsacum Dactyloides L. Hybrids and Parents. Biologia plantarum, 43(2), 211-215. doi: 10.1023/A:1002743908420
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    References

    1. Bednář, J., Provazníková, E.: [Polymorphism of enzymes as genetic markers in breeding of maize hybrids.]-Genet. Slecht (Praha) 30: 305-312, 1994. [In Czech.]
    2. Berquist, R.: Transfer from Tripsacum dactyloides to corn of major gene locus conditioning resistance to Puccinia sorghi.-Phytopathology 71: 518-520, 1981. Go to original source...
    3. Berthaud, I., Savidan, Y.: Genetic resources of Tripsacum and gene transfer to maize.-In: Mujeeb-Kazi, A., Sitch, L.A. (ed.): II International Symposium on Genetic Manipulation in Crops. Pp. 121-130. International Maize and Wheat Improvement Center, Mexico 1989.
    4. Davis, B.: Disc Electrophoresis II. Method and application to human serum proteins.-Ann. N. Y. Acad. Sci. 121: 404-427, 1964. Go to original source...
    5. Frova, G., Taramino, G., Binelli, G.: Heat-shock proteins during pollen development in maize.-Develop. Genet. 71: 324-332, 1989. Go to original source...
    6. Galinat, W.: Intergenomic mapping of maize, teosinte and Tripsacum.-Evolution 27: 644-655, 1973. Go to original source...
    7. Goodman, M., Stuber, C.: Maize.-In: Tanksley, M., Orton, T. (ed.): Isoenzymes in Plant Genetics and Breeding. Part B. Pp. 1-35. Elsevier Science Publishers, Amsterdam-New York 1983.
    8. Kindiger, B., Bai, D., Sokolov, V.: Assignment of gene(s) conferring apomixis in Tripsacum to a chromosome arm: cytological and molecular evidence.-Genome 39: 1133-1141, 1996. Go to original source...
    9. Kindiger, B., Vierlig, R.: Comparative isozyme polymorphism of North American eastern gamagrass, Tripsacum dactyloides var. dactyloides and maize, Zea mays L.-Genetica 94: 77-83, 1994. Go to original source...
    10. Leblanc, D. Grimanelli, O., Gonsalez de Leon, Savidan, J.: Detection of the apomictic mode of reproduction in maize-Tripsacum hybrids using maize RFLP markers.-Theor. appl. Genet. 90: 1198-1203, 1995. Go to original source...
    11. Magoja, I., Nivio, A.: Influence of perennial teosinte germplasm on polypeptidic patterns of maize endosperm proteins.-Maize Genet. Cooper. News Lett. 55: 62-64, 1981.
    12. Paulis, J., Wall, J.: Comparison of the protein compositions of selected corns and their wild relatives, teosinte and Tripsacum.-J. Agr. Food Chem. 25: 265-270, 1977. Go to original source...
    13. Peruanskiï, J., Savin, B.: [Method for studying zein heterogeneity in maize.]-Fiziol. Biokhim. kul't. Rast. 17: 391-393, 1985. [In Russ.]
    14. Shah, A., Stagemann, H.: Lectin based characterization of glycoproteins in plants and its application to cultivar discrimination.-Nachrichtenbl. deut. Pflanzenschutz 38: 100-103, 1986.
    15. Valentini, G., Soave, G., Ottaviano, E.: Chromosomal localization of zein genes in Zea mays.-Heredity 42: 33-40, 1979. Go to original source...
    16. Vallejos, C.: Enzyme activity staining.-In: Tanksley, M., Orton, T. (ed.): Isozymes in Plant Genetics and Breeding, Part A. Pp. 469-512. Elsevier Science Publishers, Amsterdam-New York 1983. Go to original source...
    17. Viotti, A., Pogna, N., Balducci, C., Durante, M.: Chromosomal localization of zein genes in situ hybridization in Zea mays.-Mol. gen. Genet. 178: 35-41, 1980. Go to original source...
    18. Weber, D.: Using maize monosomics to locate genes to specific chromosomes.-In: Sheridan, W. F. (ed.): Maize for Biological Research. Pp. 79-83. Plant Molecular Biology Association, Charlottesville 1982.
    19. Wet, J., Prunty, S.: Zeins as indicators of maize-Tripsacum introgression.-Biochem. Syst. Ecol. 15: 231-233, 1987. Go to original source...

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