biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 43:253-256, 2000 | DOI: 10.1023/A:1002708427984

Effect of ZnSO4 and CuSO4 on Regeneration and Lepidine Content in Lepidium Sativum L.

Saba1, D. Pande1, M. Iqbal1, P.S. Srivastava2
1 Department of Botany, Centre for Biotechnology and, New Delhi, India
2 Faculty of Science, Hamdard University, New Delhi, India

Significant amounts of lepidine was detected in mature and juvenile explants from both in vivo and in vitro grown plants. The yield, however, was variable depending upon the source and type of explant used. Mature in vivo plants at vegetative stage exhibited highest yield. Among all the explants, maximum lepidine was detected after 8 weeks in shoot apex callus on MS medium supplemented with 2 mg dm-3 naphthaleneacetic acid and 5 mg dm-3 benzylaminopurine. Addition of 900 μM Zn2- or 100 μM Cu2- further enhanced the yield of lepidine.

Keywords: differentiation; in vitro growth; medicinal plant; secondary metabolites
Subjects: copper sulphate, lepidine content; heavy metals, lepidine content; in vitro culture, differentiation, growth; in vitro culture, regeneration, lepidine content; lepidine content, heavy metals; Lepidium sativum; medicinal plants, lepidine; regeneration in vitro, lepidine content; secondary metabolites, lepidine; zinc sulphate, lepidine content

Published: August 1, 2000  Show citation

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Saba,, Pande, D., Iqbal, M., & Srivastava, P.S. (2000). Effect of ZnSO4 and CuSO4 on Regeneration and Lepidine Content in Lepidium Sativum L. Biologia plantarum, 43(2), 253-256. doi: 10.1023/A:1002708427984
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